一种用于分析百里香挥发油中百里香酚和香芹酚的经过验证的高效液相色谱法。

A validated high performance liquid chromatography method for the analysis of thymol and carvacrol in Thymus vulgaris L. volatile oil.

作者信息

Hajimehdipoor H, Shekarchi M, Khanavi M, Adib N, Amri M

机构信息

Traditional Medicine and Materia Medica Research Center and Department of Traditional Pharmacy, School of Traditional Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

出版信息

Pharmacogn Mag. 2010 Jul;6(23):154-8. doi: 10.4103/0973-1296.66927.

DOI:10.4103/0973-1296.66927

PMID:20931071

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2950374/

Abstract

Thymus vulgaris L. (Lamiaceae) is a well-known medicinal plant that contains important compounds such as thymol and carvacrol and it has been used in many pharmaceutical dosage forms. Thymol and carvacrol in essential oils are often quantified by gas chromatography (GC) technique but in this work, a validated and reliable high performance liquid chromatography (HPLC) method has been developed for the analysis of these two components in T. vulgaris essential oil. The essential oil of the plant was analyzed by HPLC and GC techniques. The HPLC system consisted of ACE C(18) column and an isocratic acetonitrile:water (50:50) as the mobile phase which was kept at a flow rate of 1 ml/min. The method was validated for selectivity, linearity (r(2) > 0.997 for both thymol and carvacrol), precision (intra-day 0.8-1.9, 1.7-2.6; and inter-day 3.5-4.5, 3.6-4.7) and recovery (97.7%, 97.6%) for thymol and carvacrol, respectively. The limits of detection (LODs) and limits of quantization (LOQs) were calculated to be 2.8, 0.6 µg/ml and 8.6, 1.8 µg/ml for thymol and carvacrol, respectively. The GC system consisted of flame ionization detector (FID) and CP-SIL 8 column. The concentrations of thymol and carvacrol in essential oil obtained by HPLC (41.2%, 4.3%) and GC (40.7%, 4.2%) were compared by statistical methods and they showed good agreement.

摘要

百里香（唇形科）是一种著名的药用植物，含有诸如百里香酚和香芹酚等重要化合物，并且已被用于多种药物剂型中。精油中的百里香酚和香芹酚通常通过气相色谱（GC）技术进行定量，但在本研究中，已开发出一种经过验证且可靠的高效液相色谱（HPLC）方法，用于分析百里香精油中的这两种成分。采用HPLC和GC技术对该植物的精油进行了分析。HPLC系统由ACE C(18)柱和乙腈:水（50:50）等度洗脱作为流动相组成，流速保持在1 ml/min。该方法在选择性、线性（百里香酚和香芹酚的r(2)均>0.997）、精密度（日内0.8 - 1.9、1.7 - 2.6；日间3.5 - 4.5、3.6 - 4.7）以及回收率（百里香酚为97.7%，香芹酚为97.6%）方面均得到验证。百里香酚和香芹酚的检测限（LODs）分别计算为2.8、0.6 µg/ml，定量限（LOQs）分别为8.6、1.8 µg/ml。GC系统由火焰离子化检测器（FID）和CP - SIL 8柱组成。通过统计学方法比较了HPLC（41.2%，4.3%）和GC（40.7%，4.2%）测得的精油中百里香酚和香芹酚的浓度，结果显示二者具有良好的一致性。