de Montigny P, Shim J S, Pivnichny J V
Merck Sharp and Dohme Research Laboratories, Rahway, NJ 07065.
J Pharm Biomed Anal. 1990;8(6):507-11. doi: 10.1016/0731-7085(90)80060-3.
Ivermectin is a potent anthelmintic agent which was detected at low concentrations in cattle plasma by LC after conversion to a fluorescent derivative. This was accomplished by reaction with acetic anhydride (AA) and pyridine for 24 h at 100 degrees C or with AA and N-methylimidazole (NMIM) for 1 h at 95 degrees C. Substituting trifluoroacetic anhydride (TFAA) for AA reduced the reaction time to less than 30 s at 25 degrees C, yielding an intensely fluorescent derivative with substantially fewer reagent by-products. The need for further sample preparation after derivatization with TFAA-NMIM was thereby eliminated, and detection limits of less than 20 pg ml-1 ivermectin could be achieved with 1 ml of plasma by a considerably simpler analytical procedure.
伊维菌素是一种强效驱虫剂，经转化为荧光衍生物后，可通过液相色谱法在牛血浆中检测到低浓度的伊维菌素。这可通过在100℃下与乙酸酐（AA）和吡啶反应24小时，或在95℃下与AA和N - 甲基咪唑（NMIM）反应1小时来实现。用三氟乙酸酐（TFAA）替代AA可将反应时间缩短至25℃下不到30秒，生成一种荧光强烈且试剂副产物显著减少的衍生物。因此，在用TFAA - NMIM衍生化后无需进一步的样品制备，通过一种相当简单的分析程序，用1毫升血浆即可实现伊维菌素检测限低于20 pg/ml。
