Universidade Estadual Paulista (UNESP), Laboratório de Fitoterápicos, Departamento de Biotecnologia, Faculdade de Ciências e Letras de Assis, Avenida Dom Antônio 2100, CEP: 19806-900 Assis, São Paulo, Brazil; Universidade Estadual Paulista (UNESP), Instituto de Química de Araraquara, Rua Prof. Francisco Degni 55, CEP: 14800-060 Araraquara, São Paulo, Brazil.
Universidade Estadual Paulista (UNESP), Instituto de Biociências de Botucatu, Departamento de Botânica, Fisiologia Vegetal, Distrito de Rubião Jr., s/n, CEP: 18618-970, Botucatu, São Paulo, Brazil.
Sci Total Environ. 2017 Dec 1;599-600:1837-1847. doi: 10.1016/j.scitotenv.2017.05.156. Epub 2017 May 20.
The eutrophication of aquatic ecosystems is a serious environmental problem that leads to increased frequency of cyanobacterial blooms and concentrations of cyanotoxins. These changes in aquatic chemistry can negatively affect animal and human health. Environment-friendly methods are needed to control bloom forming cyanobacteria. We investigated the effect of Hordeum vulgare L. (barley) straw degradation extract and its fractions on the growth, oxidative stress, antioxidant enzyme activities, and microcystins content of Microcystis aeruginosa (Kützing) Kützing BCCUSP232. Exposure to the extract significantly (p<0.05) inhibited the growth of M. aeruginosa throughout the study, whereas only the highest concentration of fractions 1 and 2 significantly (p<0.05) reduced the growth of the cyanobacterium on day 10 of the experiment. The production of reactive oxygen species (ROS), lipid peroxidation and antioxidant enzyme activities were significantly (p<0.05) altered by the extract and fractions 1 and 2. Phytochemical profiling of the extract and its fractions revealed that the barley straw degradation process yielded predominantly phenolic acids. These results demonstrate that barley straw extract and its fractions can efficiently interfere with the growth and development of M. aeruginosa under laboratory conditions.
水生态系统的富营养化是一个严重的环境问题，导致蓝藻水华的频繁发生和蓝藻毒素浓度的增加。这些水化学变化会对动物和人类健康产生负面影响。需要采用环保方法来控制形成水华的蓝藻。我们研究了大麦（Hordeum vulgare L.）秸秆降解提取物及其馏分对铜绿微囊藻（Microcystis aeruginosa （Kützing） Kützing BCCUSP232）生长、氧化应激、抗氧化酶活性和微囊藻毒素含量的影响。提取物的暴露显著（p<0.05）抑制了铜绿微囊藻的整个研究期间的生长，而仅在实验第 10 天，馏分 1 和 2 的最高浓度才显著（p<0.05）降低了蓝藻的生长。提取物和馏分 1 和 2 显著（p<0.05）改变了活性氧（ROS）的产生、脂质过氧化和抗氧化酶活性。提取物及其馏分的植物化学特征分析表明，大麦秸秆降解过程主要产生了酚酸。这些结果表明，大麦秸秆提取物及其馏分可以在实验室条件下有效地干扰铜绿微囊藻的生长和发育。
