Department of Lipid Science and Traditional Foods, Central Food Technological Research Institute, Council of Scientific and Industrial Research, Mysore, India.
Food Chem Toxicol. 2011 Jan;49(1):136-43. doi: 10.1016/j.fct.2010.10.008. Epub 2010 Oct 15.
The present study was undertaken to evaluate the effect of feeding blended and interesterified oils prepared using coconut oil (CNO) with rice bran oil (RBO) or sesame oil (SESO), with a polyunsaturated/saturated (P/S) ratio of 0.8-1.0, on oxidative stress and endogenous antioxidant system. Feeding blended oils resulted in significantly increased hepatic lipid peroxide levels in rats given blended oil CNO+RBO or CNO+SESO by 1.3 and 1.6-fold, respectively compared to rats fed diet containing CNO. The lipid peroxide level in erythrocyte membrane also increased in rats fed blended oil compared to rats fed with CNO. Rats fed interesterified oils prepared from these blended oils also showed increased lipid peroxide level compared to rats given CNO diet, however it was not significantly different from rats fed with their respective blends. There was a significant increase in the activity of endogenous antioxidant enzymes super oxide dismutase, catalase, glutathione peroxidase and glutathione-s-transferase after feeding blended and interesterified oils. The activities of Na(+)/K(+)-ATPase and Ca(2+)/Mg(2+)-ATPase were increased in rats fed blended and interesterified oils. These results indicated that the P/S ratio of dietary fat is an important factor in determining the oxidative stress, activity of endogenous antioxidant enzymes and activity of membrane bound enzymes.
本研究旨在评估以椰子油（CNO）与米糠油（RBO）或芝麻油（SESO）混合并进行酯交换制成的、具有 0.8-1.0 的多不饱和/饱和（P/S）比值的混合及交酯油对氧化应激和内源性抗氧化系统的影响。与给予 CNO 饮食的大鼠相比，给予混合油 CNO+RBO 或 CNO+SESO 的大鼠的肝脂质过氧化物水平分别显著增加了 1.3 倍和 1.6 倍。与给予 CNO 的大鼠相比，给予混合油的大鼠的红细胞膜脂质过氧化物水平也增加了。与给予 CNO 饮食的大鼠相比，用这些混合油制备的交酯油喂养的大鼠的脂质过氧化物水平也有所增加，但与给予相应混合物的大鼠相比没有显著差异。给予混合和交酯油后，内源性抗氧化酶超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽 S-转移酶的活性显著增加。给予混合和交酯油后，Na(+)/K(+)-ATP 酶和 Ca(2+)/Mg(2+)-ATP 酶的活性增加。这些结果表明，膳食脂肪的 P/S 比值是决定氧化应激、内源性抗氧化酶活性和膜结合酶活性的重要因素。
