Department of Medical Microbiology, Lund University, Sölvegatan 23, SE-223 62 Lund, Sweden.
World J Gastroenterol. 2010 Oct 14;16(38):4817-22. doi: 10.3748/wjg.v16.i38.4817.
To analyze the association between Helicobacter spp. and some common gut bacteria in patients with cholecystitis.
A nested-polymerase chain reaction (PCR), specific to 16S rRNA of Helicobacter spp. was performed on paraffin-embedded gallbladder samples of 100 cholecystitis and 102 control cases. The samples were also analyzed for some common gut bacteria by PCR. Positive samples were sequenced for species identification.
Helicobacter DNA was found in seven out of 100 cases of acute and chronic cholecystitis. Sequence analysis displayed Helicobacter pullorum (H. pullorum) in six cases and Helicobacter pylori in one; H. pullorum was only found in cases with metaplasia. Control samples were negative for Helicobacter spp. and some common gut bacteria. There was a significant difference (P = 0.007) between cholecystitis and control samples for Helicobacter DNA.
A possible relationship was detected between Helicobacter DNA and cholecystitis. Further serological and immunohistochemical studies are needed to support these data.
分析胆石症患者中幽门螺杆菌属与一些常见肠道细菌之间的关联。
对 100 例胆石症和 102 例对照病例的石蜡包埋胆囊样本进行巢式聚合酶链反应（PCR），针对幽门螺杆菌属 16S rRNA 进行特异性检测。还通过 PCR 对一些常见肠道细菌进行了分析。对阳性样本进行测序以确定物种。
在 100 例急性和慢性胆囊炎中，有 7 例发现了幽门螺杆菌属 DNA。序列分析显示，6 例为鸡幽门螺杆菌（H. pullorum），1 例为幽门螺杆菌；H. pullorum 仅在化生病例中发现。对照样本均未检出幽门螺杆菌属和一些常见肠道细菌。幽门螺杆菌属 DNA 在胆石症和对照组之间存在显著差异（P = 0.007）。
检测到幽门螺杆菌属 DNA 与胆石症之间可能存在关联。需要进一步的血清学和免疫组织化学研究来支持这些数据。
