Department of Gastroenterology, Miyoshi Central Hospital, Miyoshi, Hiroshima, Japan.
Helicobacter. 2009 Dec;14(6):545-51. doi: 10.1111/j.1523-5378.2009.00729.x.
Since the discovery of Helicobacter pylori, various enterohepatic Helicobacter spices have been detected in the guts of humans and animals. Some enterohepatic Helicobacters have been associated with inflammatory bowel disease or liver disease in mice. However the association of these bacteria with human diseases remains unknown.
We collected 126 bile samples from patients with cholelithiasis, cholecystitis, gallbladder polyp, and other nonbiliary diseases. Samples were screened for the presence of enterohepatic Helicobacter spp. using cultures, nested PCR, or in situ hybridization. We tested for antibodies to H. pylori and H. hepaticus by Western blot analysis.
Attempts at cultivation were unsuccessful. However, H. hepaticus was detected in bile samples with nested PCR whereas H. bilis was not. Helicobacter hepaticus in the bile was confirmed by in situ hybridization, but H. hepaticus from bile samples was coccoid in appearance. We detected immunoglobulin G antibodies to H. hepaticus in bile samples by Western blotting. Helicobacter hepaticus was detected in 40 (32%) of total 126 samples as H. hepaticus positive if at least one of the three methods with nested PCR, in situ, or Western blotting. Patients with cholelithiasis (41%) and cholecystitis with gastric cancer (36%) had significantly higher (p = .029) prevalence of H. hepaticus infection than samples from patients with other diseases.
Helicobacter hepaticus may closely associate with diseases of the liver and biliary tract in humans.
自幽门螺杆菌被发现以来，人类和动物的肠道中已检测到多种肠肝幽门螺杆菌属。一些肠肝幽门螺杆菌与小鼠的炎症性肠病或肝病有关。然而，这些细菌与人类疾病的关联尚不清楚。
我们收集了 126 份来自胆结石、胆囊炎、胆囊息肉和其他非胆道疾病患者的胆汁样本。使用培养、巢式 PCR 或原位杂交法筛查肠肝幽门螺杆菌属的存在。我们通过 Western blot 分析测试了针对 H. pylori 和 H. hepaticus 的抗体。
培养尝试均未成功。然而，巢式 PCR 检测到胆汁样本中存在 H. hepaticus，而 H. bilis 则不存在。胆汁中的 H. hepaticus 通过原位杂交得到确认，但胆汁样本中的 H. hepaticus 呈球形外观。我们通过 Western blot 在胆汁样本中检测到针对 H. hepaticus 的 IgG 抗体。通过巢式 PCR、原位或 Western blot 三种方法中的至少一种检测到 H. hepaticus 的胆汁样本为 40 份（32%），如果至少有一种方法检测到 H. hepaticus，则为 H. hepaticus 阳性。胆结石（41%）和伴有胃癌的胆囊炎（36%）患者的 H. hepaticus 感染率明显高于其他疾病患者的样本（p =.029）。
H. hepaticus 可能与人类肝脏和胆道疾病密切相关。
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