Division of Oral Biology, Schulich School of Medicine and Dentistry, Dental Sciences Building, The University of Western Ontario, London, Ontario, Canada.
J Dent Res. 2010 Dec;89(12):1439-43. doi: 10.1177/0022034510378684. Epub 2010 Oct 12.
Recently identified as a key component of the murine periodontal ligament (PDL), periostin has been implicated in the regulation of collagen fibrillogenesis and fibroblast differentiation. We investigated whether periostin protein is expressed in the human PDL in situ and the mechanisms regulating periostin expression in PDL fibroblasts in vitro. With immunohistochemistry, periostin protein was identified in the PDL, with expression lower in teeth with reduced occlusal loading. In vitro application of uniaxial cyclic strain to PDL fibroblasts elevated periostin mRNA levels, depending on the age of the patient. Treatment with transforming growth factor-beta1 (TGF-β1) also significantly increased periostin mRNA levels, an effect attenuated by focal adhesion kinase (FAK) inhibition. FAK-null fibroblasts contained no detectable periostin mRNA, even after stimulation with cyclic strain. In conclusion, periostin protein is strongly expressed in the human PDL. In vitro, periostin mRNA levels are modulated by cyclic strain as well as TGF-β1 via FAK-dependent pathways.
最近被鉴定为小鼠牙周韧带（PDL）的关键组成部分，骨膜蛋白被认为参与了胶原原纤维生成和成纤维细胞分化的调节。我们研究了骨膜蛋白蛋白是否在人牙周韧带原位表达，以及体外调节牙周韧带成纤维细胞中骨膜蛋白表达的机制。通过免疫组织化学染色，在牙周韧带中鉴定出了骨膜蛋白，在咬合负荷降低的牙齿中表达水平较低。体外应用单轴循环应变可升高牙周韧带成纤维细胞的骨膜蛋白 mRNA 水平，且这一效果取决于患者的年龄。转化生长因子-β1（TGF-β1）处理也显著增加了骨膜蛋白 mRNA 水平，这一效应可被粘着斑激酶（FAK）抑制所减弱。FAK 缺陷型成纤维细胞即使在受到循环应变刺激后，也检测不到可检测到的骨膜蛋白 mRNA。总之，骨膜蛋白蛋白在人牙周韧带中强烈表达。在体外，骨膜蛋白 mRNA 水平可通过循环应变以及 TGF-β1 通过 FAK 依赖途径进行调节。
