Phylogenetics Laboratory, BIOTEC, NSTDA Science Park, 113 Paholyothin Road, Klong 1, Klong Luang, Pathum Thani 12120, Thailand.
Fungal Biol. 2010 Sep;114(9):739-45. doi: 10.1016/j.funbio.2010.06.007. Epub 2010 Jun 15.
A hypocrealean Coleoptera pathogen with characteristic part-spores, collected from Khao Yai National Park and Kaeng Krachan National Park in Thailand, is reported. The overall morphology was similar to Cordyceps barnesii, which is known from Sri Lanka, with ascospores disarticulating into four unusually long part-spores that were 30-40 μm long. This disarticulation and part-spore size is, so far, unique within Cordyceps sensu lato. The Thai material was identified with C. barnesii and its placement in the genus Ophiocordyceps was confirmed. Multigene analyses based on the ribosomal small subunit, RPB1 and RPB2 genes revealed the close relationship of the Thai material to Ophiocordyceps konnoana as well as O. ravenelii, O. superficialis, and O. nigrella (all of which have significantly smaller part-spores). However, Ophiocordyceps barnesii and these related species were all characterised by dark-brown to purplish stromata and an affinity for melolonthid larval hosts. No anamorph was seen in the field and was not produced in the slow-growing cultures.
报道了一种从泰国考艾国家公园和干拉昌国家公园采集的拟青霉科病原菌，其特征为部分孢子。其整体形态与已知自斯里兰卡的巴氏虫草相似，子囊孢子解体成四个异常长的部分孢子，长 30-40μm。这种解体和部分孢子大小在广义虫草属中是独一无二的。泰国材料被鉴定为巴氏虫草，其在 Ophiocordyceps 属中的位置得到了确认。基于核糖体小亚基、RPB1 和 RPB2 基因的多基因分析表明，泰国材料与 Ophiocordyceps konnoana 以及 O. ravenelii、O. superficialis 和 O. nigrella（所有这些都具有明显更小的部分孢子）密切相关。然而，巴氏虫草和这些相关物种的特征都是深褐色到紫色的子实体，以及对金龟子幼虫宿主的亲和力。在野外未观察到无性型，在缓慢生长的培养物中也未产生。
