Ristuccia P A, Ristuccia A M, Bidanset J H, Cunha B A
Ther Drug Monit. 1984;6(2):238-42. doi: 10.1097/00007691-198406000-00019.
This investigation was designed to compare three assay techniques, the traditional bioassay (agar diffusion), and two more recent techniques, high-performance liquid chromatography (HPLC) and fluorescence polarization immunoassay (FPIA), for the determination of vancomycin concentrations in serum. One hundred clinical samples obtained from patients receiving vancomycin were assayed by each method. The results from each assay were compared using linear regression analysis. The resultant correlation coefficients were as follows: 0.9996 for the HPLC versus FPIA, 0.7773 for the FPIA versus bioassay, and 0.7779 for HPLC versus bioassay. The FPIA technique was the easiest and fastest of the three methods; FPIA and HPLC were the most accurate.
本研究旨在比较三种检测技术，即传统生物测定法（琼脂扩散法）以及两种较新的技术，高效液相色谱法（HPLC）和荧光偏振免疫分析法（FPIA），用于测定血清中的万古霉素浓度。对从接受万古霉素治疗的患者中获取的100份临床样本分别采用每种方法进行检测。使用线性回归分析对每种检测结果进行比较。所得相关系数如下：HPLC与FPIA之间为0.9996，FPIA与生物测定法之间为0.7773，HPLC与生物测定法之间为0.7779。FPIA技术是三种方法中最简单、最快的；FPIA和HPLC最为准确。
