Division of Pharmacognosy, College of Pharmacy, Kyung Hee University, Seoul, 130-701, Korea.
Arch Pharm Res. 2010 Sep;33(9):1355-9. doi: 10.1007/s12272-010-0909-7. Epub 2010 Oct 9.
This study was conducted to provide a more versatile and specific information on Thin Layer Chromatographic (TLC) analysis of medicinal plants. TLC plates developed with the extract of herbal medicines were analyzed with direct analysis in real time (DART) ion source. Three well known herbal drugs were extracted and developed on a silica-coated TLC plate with the conditions pre-established in Korean Pharmacopoeia IX. The developed plate was placed between the DART ion source and TOF-MS analyzer to get real time mass spectra from the bands on the TLC plate directly. The marker coumarin compounds, decursin and decursinol were successfully identified from the TLC plate developed with Angelicae gigantis radix, along with alkaloid compounds of rutaecarpine and evodiamine from Evodiae fructus, and lignan molecules of gomisin A, N, and schisandrin from Schisandrae fructus. This hyphenation system of TLC and DART-MS could provide unique and specific information on the major constituents of crude plant drug on TLC through uncovering high resolution mass number of each band on the TLC plate directly in real time.
本研究旨在提供更通用和具体的信息，介绍中药材的薄层色谱（TLC）分析。使用直接实时分析（DART）离子源分析用草药提取物制备的 TLC 板。三种知名草药用韩国药典 IX 中预先确定的条件提取并在涂覆有硅胶的 TLC 板上展开。将展开的板放置在 DART 离子源和 TOF-MS 分析仪之间，以便直接从 TLC 板上的条带获得实时质谱。从独活根制备的 TLC 板上成功鉴定出标记香豆素化合物当归素和当归醇，以及吴茱萸中的生物碱化合物吴茱萸碱和吴茱萸碱，以及五味子中的木脂素分子五味子甲素、五味子乙素和五味子丙素。TLC 和 DART-MS 的这种连接系统可以通过直接实时揭示 TLC 板上每个条带的高分辨率质量数，为 TLC 上粗植物药的主要成分提供独特而具体的信息。
