College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.
Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing, China.
PLoS One. 2018 Apr 6;13(4):e0195240. doi: 10.1371/journal.pone.0195240. eCollection 2018.
The accurate identification and quality evaluation of herbal medical plants is highly necessary to ensure their safety and efficacy. In present study, a new strategy combining DNA barcoding techniques with thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC) was proposed to facilitate the identification and quality control of M. tenacissima. In present work, the internal transcribed spacer 2 (ITS2) barcode was successfully used to identify 58 M. tenacissima samples and its adulterants. TLC successfully identified the other three M. tenacissima samples that failed to produce ITS2 regions. An adulterant was found in all the 62 samples. Moreover, the content of active medicinal ingredients is important for herbal plants quality. The content of tenacissoside H (TS-H) of M. tenacissima samples was determined by HPLC to range from 0.39% to 1.09%, which meets the criterion of the Chinese Pharmacopoeia. Thus, DNA barcoding coupled with TLC and HPLC is very promising to identify and evaluate the quality of M. tenacissima in the medicine market.
准确鉴定和质量评估药用植物是非常必要的，以确保其安全性和疗效。在本研究中，提出了一种结合 DNA 条形码技术与薄层色谱（TLC）和高效液相色谱（HPLC）的新策略，以促进对菝葜的鉴定和质量控制。在本工作中，成功地使用内转录间隔区 2（ITS2）条形码鉴定了 58 份菝葜及其掺杂物。TLC 成功地鉴定了另外三个未能产生 ITS2 区的菝葜样本。在所有 62 个样本中都发现了掺杂物。此外，活性药用成分的含量对草药质量很重要。采用高效液相色谱法测定菝葜样品中菝葜皂苷 H（TS-H）的含量为 0.39%至 1.09%，符合中国药典标准。因此，DNA 条形码结合 TLC 和 HPLC 非常有希望鉴定和评估医药市场上菝葜的质量。
