Bouck N, Beales N, Shenk T, Berg P, di Mayorca G
Proc Natl Acad Sci U S A. 1978 May;75(5):2473-7. doi: 10.1073/pnas.75.5.2473.
Viable mutants of simian virus 40 with deletions in three regions of the virus genome (map coordinates 0.21-0.17, 0.59-0.54, and 0.67-0.74) have been tested for their ability to transform rat fibroblasts to anchorage independence. Only those mutants whose deletions occur between 0.59 and 0.55 in the proximal part of the early region are defective in transforming ability. The most severely defective of these transform with less than one-hundredth the efficiency of wild type. They retain their defect when tested in Chinese hamster lung cells and when infection is initiated with viral DNA instead of intact virions. Complementation for transformation can be observed between these transformation-defective deletions and a simian virus 40 temperature-sensitive A mutant.
对猿猴病毒40在病毒基因组三个区域(图谱坐标0.21 - 0.17、0.59 - 0.54和0.67 - 0.74)发生缺失的存活突变体进行了测试,以检测它们将大鼠成纤维细胞转化为不依赖贴壁生长的能力。只有那些在早期区域近端0.59至0.55之间发生缺失的突变体在转化能力上存在缺陷。其中缺陷最严重的突变体转化效率不到野生型的百分之一。当在中国仓鼠肺细胞中进行测试以及用病毒DNA而非完整病毒粒子引发感染时,它们仍保持缺陷状态。在这些转化缺陷型缺失突变体与猿猴病毒40温度敏感型A突变体之间可观察到转化互补现象。