Sompayrac L, Danna K J
Mol Cell Biol. 1985 May;5(5):1191-4. doi: 10.1128/mcb.5.5.1191-1194.1985.
F8dl is a simian virus 40 early-region deletion mutant that lacks the sequences between 0.169 and 0.423 map units. We show that cloned F8dl DNA immortalized early-passage Fisher rat embryo cells with an efficiency that was about 20% of that of cloned wild-type simian virus 40 DNA. In contrast, we detected no immortalized colonies when we transfected the cells with DNA of five other early-region deletion mutants that do not make stable truncated forms of T antigen. Since all five of these mutants have intact early- and late-region control sequences, we conclude that these control sequences are not sufficient for immortalization. Three of the mutants that did not immortalize did make a normal small t antigen, suggesting that the expression of this protein alone is not sufficient for immortalization of early-passage Fisher rat embryo cells.
F8dl是一种猿猴病毒40早期区域缺失突变体，它缺少0.169至0.423个图距单位之间的序列。我们发现，克隆的F8dl DNA使早期传代的Fisher大鼠胚胎细胞永生化，其效率约为克隆的野生型猿猴病毒40 DNA的20%。相比之下，当我们用其他五个不产生稳定截短形式T抗原的早期区域缺失突变体的DNA转染细胞时，未检测到永生化菌落。由于这五个突变体都具有完整的早期和晚期区域控制序列，我们得出结论，这些控制序列不足以实现永生化。三个未实现永生化的突变体确实产生了正常的小t抗原，这表明仅该蛋白的表达不足以使早期传代的Fisher大鼠胚胎细胞永生化。
