Hosseinimehr Seyed Jalal, Azadbakht Mohammad, Tanha Mohammad, Mahmodzadeh Aziz, Mohammadifar Sohila
Department of Radiopharmacy, Faculty of Pharmacy, Traditional and Complementary Medicine Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Toxicol Ind Health. 2011 May;27(4):363-9. doi: 10.1177/0748233710387010. Epub 2010 Oct 14.
The preventive effect of hawthorn (Crataegus microphylla) fruit extract against genotoxicity induced by methyl methanesulfonate (MMS) has been investigated in human cultured blood lymphocytes. Peripheral blood samples were collected from human volunteers at 0 (10 minutes before), and at 1 and 2 hours after a single oral ingestion of 1 g hawthorn powder extract. At each time point, the whole blood was treated in vitro with MMS (200 µmol) at 24 hours after cell culture, and then the lymphocytes were cultured with mitogenic stimulation to determine the micronuclei in cytokinesis-blocked binucleated cells. The lymphocytes treated with hawthorn and MMS to exhibit a significant decreasing in the incidence of micronucleated binucleated cells, as compared with similarly MMS-treated lymphocytes from blood samples collected at 0 hour. The maximum protection and decreasing in frequency of micronuclei (36%) was observed at 1 hour after ingestion of hawthorn extract. The high performance liquid chromatography (HPLC) analysis showed that hawthorn contained chlorogenic acid, epicatechin and hyperoside. It is obvious that hawthorn, particularly flavonoids constituents with antioxidative activity, reduced the oxidative stress and genotoxicity induced by toxic compounds. This set of data may have an important application for the protection of human lymphocyte from the genetic damage and side effects induced by chemicals hazardous in people.
在人类培养的血液淋巴细胞中，研究了山楂（小叶山楂）果实提取物对甲磺酸甲酯（MMS）诱导的遗传毒性的预防作用。从人类志愿者中采集外周血样本，分别在单次口服1 g山楂粉提取物前0小时（10分钟）、1小时和2小时采集。在每个时间点，细胞培养24小时后，全血在体外与MMS（200 µmol）处理，然后用有丝分裂原刺激培养淋巴细胞，以确定胞质分裂阻滞双核细胞中的微核。与在0小时采集的血样中同样经MMS处理的淋巴细胞相比，经山楂和MMS处理的淋巴细胞微核双核细胞发生率显著降低。摄入山楂提取物1小时后观察到最大程度的保护和微核频率降低（36%）。高效液相色谱（HPLC）分析表明，山楂含有绿原酸、表儿茶素和金丝桃苷。显然，山楂，特别是具有抗氧化活性的黄酮类成分，减少了有毒化合物诱导的氧化应激和遗传毒性。这组数据对于保护人类淋巴细胞免受化学有害物质诱导的遗传损伤和副作用可能具有重要应用。
