Renda Gülin, Özel Arzu, Barut Burak, Korkmaz Büşra, Yayli Nurettin
Karadeniz Technical University, Faculty of Pharmacy, Department of Pharmacognosy, Trabzon, Turkey.
Karadeniz Technical University, Faculty of Pharmacy, Department of Biochemistry, Trabzon, Turkey.
Turk J Pharm Sci. 2018 Apr;15(1):77-84. doi: 10.4274/tjps.39200. Epub 2018 Apr 2.
species have been used as food and also in folk medicine for the treatment of various diseases. The present study aimed to make investigations on the biologic properties of different extracts prepared from C. Koch, which was collected from Turkey.
Dried leaf, stem bark, and fresh fruit samples of were separated and ethanol extract, acidified (0.5% HCl, pH: 2.5) ethanol extract, ethanol:water (1:1) extract, methanol extract, acidified (0.5% HCl, pH: 2.5) methanol extract, methanol:water (1:1) extract, water extract, and acidified (0.5% HCl, pH: 2.5) water extract were prepared for each. Various biologic effects such as the prevention of oxidative DNA damage, acetylcholinesterase, tyrosinase, α-glucosidase inhibition, and antioxidant effects with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, PRAP, and FRAP assays of these extracts at different concentrations were studied.
Acidified methanol extract of stem barks exhibited the highest acetylcholinesterase and tyrosinase inhibitions among the other extracts with IC values of 204.02±0.95 μg/mL and 37.30±0.27 μg/mL, respectively. Acidified ethanol extract of leaves was the most efficient extract against α-glucosidase, giving an IC of 15.78±0.14 μg/mL. The IC value of the acidified ethanol extract for DPPH was 9.89±0.09 µg/mL. Methanol extracts of leaves and stem barks at the dose of 125 µg/mL exhibited significant protective activity against DNA strand scission by hydroxyl radicals (.OH) on supercoiled pBR322 DNA.
Acidified methanol or ethanol extracts prepared with stem bark and leaf from have potential antioxidant, hypoglycemic, and neuroprotective effects.
某些物种既被用作食物，也被用于民间医学治疗各种疾病。本研究旨在对从土耳其采集的科赫氏[物种名称未完整给出]制备的不同提取物的生物学特性进行研究。
分离[物种名称未完整给出]的干燥叶片、茎皮和新鲜果实样本，分别制备乙醇提取物、酸化（0.5%盐酸，pH：2.5）乙醇提取物、乙醇：水（1:1）提取物、甲醇提取物、酸化（0.5%盐酸，pH：2.5）甲醇提取物、甲醇：水（1:1）提取物、水提取物和酸化（0.5%盐酸，pH：2.5）水提取物。研究了这些提取物在不同浓度下的各种生物学效应，如预防氧化性DNA损伤、乙酰胆碱酯酶、酪氨酸酶、α - 葡萄糖苷酶抑制作用，以及通过2,2 - 二苯基 - 1 - 苦基肼（DPPH）自由基清除、PRAP和FRAP测定的抗氧化作用。
茎皮的酸化甲醇提取物在其他提取物中表现出最高的乙酰胆碱酯酶和酪氨酸酶抑制作用，IC值分别为204.02±0.95μg/mL和37.30±0.27μg/mL。叶片的酸化乙醇提取物是对抗α - 葡萄糖苷酶最有效的提取物，IC值为15.78±0.14μg/mL。酸化乙醇提取物对DPPH的IC值为9.89±0.09μg/mL。叶片和茎皮的甲醇提取物在125μg/mL剂量下对超螺旋pBR322 DNA上的羟基自由基（·OH）引起的DNA链断裂表现出显著的保护活性。
用[物种名称未完整给出]的茎皮和叶片制备的酸化甲醇或乙醇提取物具有潜在的抗氧化、降血糖和神经保护作用。
