Department of Surgery, Institute of Health Biosciences, The University of Tokushima Graduate School, Tokushima, Japan.
J Surg Res. 2012 Mar;173(1):180-4. doi: 10.1016/j.jss.2010.09.011. Epub 2010 Oct 8.
A new refrigerating system has been recently developed, which can cool the inside of a material to the required temperature by frequently sensing the temperature of both the inside and the surface of the materials. The purpose of this study was to evaluate the usefulness of this new system in whole blood preservation.
Study 1 (human blood): Whole blood samples from healthy volunteers were anticoagulated with CPDA. The samples were divided into two groups. Group A, whole blood preserved in ordinary refrigerator and Group B, whole blood preserved in new refrigerator (4°C, 15 wk). After preservation, ammonia, lactate, pH. and ATP levels were analyzed. Study 2 (rat experiment): Whole blood samples from male Wistar rats, stored in ordinary refrigerator (Group C) or new refrigerator (Group D). Whole blood was transfused after 8-wk storage and survival was compared.
Study 1: The ammonia concentrations in Group B were significantly lower than those in Group A at 7 and 9 wk after preservation (P < 0.05). The lactate levels in Group B were significantly lower than those in Group A at 9 wk after preservation (P < 005). The pH levels in Group B were higher than those in Group A at 5 to 13 wk after preservation (P < 0.05). The ATP levels in Group B were higher than those in Group A at 5 to 13 wk after preservation. Study 2: In rat studies using blood transfusion in acute hemorrhagic shock model, survival rate in Group C was significantly higher than those in Group D (P < 0.05).
Our new refrigerator is useful in preserving whole blood with good condition during preservation.
最近开发了一种新的制冷系统，该系统可以通过频繁感测材料内部和表面的温度，将材料内部冷却到所需的温度。本研究的目的是评估该新系统在全血保存中的有用性。
研究 1（人血）：用 CPDA 抗凝来自健康志愿者的全血样本。将样本分为两组。A 组，普通冰箱保存的全血；B 组，新冰箱（4°C，15 周）保存的全血。保存后，分析氨、乳酸、pH 值和 ATP 水平。研究 2（大鼠实验）：来自雄性 Wistar 大鼠的全血样本，储存在普通冰箱（C 组）或新冰箱（D 组）中。储存 8 周后输注全血并比较存活情况。
研究 1：保存后 7 和 9 周，B 组的氨浓度明显低于 A 组（P<0.05）。保存后 9 周，B 组的乳酸水平明显低于 A 组（P<0.05）。B 组的 pH 值在保存后 5 至 13 周高于 A 组（P<0.05）。保存后 5 至 13 周，B 组的 ATP 水平高于 A 组。研究 2：在急性失血性休克模型中使用输血的大鼠研究中，C 组的存活率明显高于 D 组（P<0.05）。
我们的新型冰箱在保存期间可保持全血良好状态，具有实用性。
