Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
Exp Gerontol. 2011 Jan;46(1):81-5. doi: 10.1016/j.exger.2010.10.002. Epub 2010 Oct 14.
We performed proteomic analysis of plasma proteins in Japanese semisuper centenarians (SSCs) (>105 years) and young controls (20-39 years), and found that 18 protein spots were altered in the plasma of SSCs. From peptide mass fingerprinting following in-gel digestion, it was demonstrated that paraoxonase/arylesterase 1 (PON 1) and apolipoprotein E were decreased, while haptoglobin β-chain, α(1)-microglobulin, and clusterin precursor were increased in SSCs. Interestingly, proteins related to oxidative stress, PON1, haptoglobin, α(1)-microglobulin, and clusterin, were altered in SSCs. These results suggest that systemic redox regulation is important for the longevity of SSCs. Overall, proteomics analysis is a powerful technique to search for useful biomarkers for future studies in gerontology and to characterize the individual proteins associated with successful aging of SSCs.
我们对日本超长龄老人（SSC，年龄>105 岁）和年轻对照组（20-39 岁）的血浆蛋白进行了蛋白质组学分析，发现 SSC 血浆中有 18 个蛋白点发生了改变。通过胶内消化后的肽质量指纹图谱分析，证实 SSC 中对氧磷酶/芳酯酶 1（PON1）和载脂蛋白 E 减少，而触珠蛋白β链、α1-微球蛋白和前簇蛋白增加。有趣的是，与氧化应激、PON1、触珠蛋白、α1-微球蛋白和聚集素相关的蛋白质在 SSC 中发生了改变。这些结果表明，全身氧化还原调节对 SSC 的长寿很重要。总的来说，蛋白质组学分析是一种强大的技术，可以为老年学的未来研究寻找有用的生物标志物，并对与 SSC 成功衰老相关的个体蛋白进行特征描述。
