Venkata Ramana Vemuluri, Sasikala Chintalapati, Veera Venkata Ramaprasad Eedara, Venkata Ramana Chintalapati
Bacterial Discovery Laboratory, Centre for Environment, Institute of Science and Technology, J.N.T. University Hyderabad, Kukakatpally, Hyderabad, India.
J Gen Appl Microbiol. 2010 Aug;56(4):313-9. doi: 10.2323/jgam.56.313.
Strain JA430(T) is a Gram-negative, vibrioid to spiral shaped phototrophic purple sulfur bacterium isolated from anoxic sediment of a saltern at Kanyakumari in a mineral salts medium that contained 2% NaCl (w/v). Strain JA430(T) grows optimally at 5-6% NaCl and tolerates up to 12% NaCl. Intracellular photosynthetic membranes were of the lamellar type. Bacteriochlorophyll a and carotenoids of the spirilloxanthin series are present as photosynthetic pigments. Major cellular fatty acids are C(18:1)ω7c, C(16:0), C(19:0)cycloω8c and C(16:1)ω7c/C(16:1)ω6c. Strain JA430(T) exhibits photoorganoheterotrophy and chemoorganoheterotrophy and requires para-aminobenzoic acid, pantothenate and pyridoxal phosphate for growth. Phylogenetic analysis on the basis of 16S rRNA gene sequence analysis showed that strain JA430(T) forms monophyletic group in the genus Ectothiorhodospira. The highest sequence similarity for strain JA430(T) was found with the type strains of Ectothiorhodospira variabilis DSM 21381(T) (96.1%) and Ectothiorhodospira haloalkaliphila ATCC 51935(T) (96.2%). Morphological and physiological characteristics discriminate strain JA430(T) from other species of the genus Ectothiorhodospira, for which we describe this as a novel species, Ectothiorhodospira salini sp. nov. ( = NBRC 105915(T) = KCTC 5805(T)).
菌株JA430(T)是一种革兰氏阴性、弧状至螺旋状的光合紫色硫细菌，从印度坎亚库马里一个盐场的缺氧沉积物中，在含有2% NaCl（w/v）的矿物盐培养基中分离得到。菌株JA430(T)在5-6% NaCl浓度下生长最佳，可耐受高达12% NaCl。细胞内光合膜为层状类型。螺旋藻黄素系列的细菌叶绿素a和类胡萝卜素作为光合色素存在。主要细胞脂肪酸为C(18:1)ω7c、C(16:0)、C(19:0)环ω8c和C(16:1)ω7c/C(16:1)ω6c。菌株JA430(T)表现出光能有机异养和化能有机异养，生长需要对氨基苯甲酸、泛酸盐和磷酸吡哆醛。基于16S rRNA基因序列分析的系统发育分析表明，菌株JA430(T)在嗜盐红螺菌属中形成一个单系群。菌株JA430(T)与可变嗜盐红螺菌DSM 21381(T)（96.1%）和嗜盐碱嗜盐红螺菌ATCC 51935(T)（96.2%）的模式菌株具有最高的序列相似性。形态和生理特征将菌株JA430(T)与嗜盐红螺菌属的其他物种区分开来，因此我们将其描述为一个新物种，盐嗜盐红螺菌（Ectothiorhodospira salini sp. nov.）（= NBRC 105915(T) = KCTC 5805(T)）。
