Guyoneaud Rémy, Mouné Sophie, Eatock Claire, Bothorel Virginie, Hirschler-Réa Agnès, Willison John, Duran Robert, Liesack Werner, Herbert Rodney, Matheron Robert, Caumette Pierre
Laboratoire d'Ecologie Moléculaire - Microbiologie, IBEAS, Université de Pau, BP 1155, 64013, Pau Cedex 13, France.
Arch Microbiol. 2002 Nov;178(5):315-24. doi: 10.1007/s00203-002-0454-y. Epub 2002 Jul 26.
Three new spirilloid phototrophic purple nonsulfur bacteria were isolated in pure culture from three different environments: strain CE2105 from a brackish lagoon in the Arcachon Bay (Atlantic coast, France), strain SE3104 from a saline sulfur spring in the Pyrenees (Navarra, Spain), and strain AT2115 a microbial mat (Tetiaroa Atoll, Society Islands). Single cells of the three strains were spiral-shaped and highly motile. Their intracellular photosynthetic membranes were of the vesicular type. Bacteriochlorophyll a and carotenoids of the normal spirilloxanthin series were present as photosynthetic pigments. Optimal growth occurred under photoheterotrophic conditions and in the presence of 0.5-4% w/v NaCl. These features are similar to those described for Roseospira mediosalina. Comparative sequence analysis of their 16S rRNA genes placed these strains within the alpha-subclass of Proteobacteria, in a cluster together with Roseospira mediosalina and Rhodospira trueperi. They form a closely related group of slightly to moderately halophilic spiral-shaped purple nonsulfur bacteria.However, the three new isolates exhibited some differences in their physiology and genetic characteristics. Consequently, we propose that they are members of three new species within the genus Roseospira, Roseospira marina sp. nov., Roseospira navarrensis sp. nov., and Roseospira thiosulfatophila sp. nov., with strains CE2105, SE3104, and AT2115 as the type strains, respectively. As a consequence, an emended description of the genus Roseospira is also given.
CE2105菌株来自阿卡雄湾（法国大西洋海岸）的咸淡水泻湖，SE3104菌株来自比利牛斯山脉（西班牙纳瓦拉）的盐性硫磺泉，AT2115菌株来自微生物垫（社会群岛的泰蒂亚罗阿环礁）。这三株菌株的单细胞呈螺旋状且运动性极强。它们的细胞内光合膜属于囊泡型。正常螺旋藻黄素系列的细菌叶绿素a和类胡萝卜素作为光合色素存在。在光异养条件下且存在0.5 - 4% w/v氯化钠时生长最佳。这些特征与中盐玫瑰螺菌所描述的特征相似。对其16S rRNA基因进行的比较序列分析将这些菌株置于变形菌门的α亚类中，与中盐玫瑰螺菌和特鲁珀红螺菌聚为一个簇。它们形成了一组密切相关的轻度至中度嗜盐的螺旋状紫色非硫细菌。然而，这三株新分离株在生理和遗传特征上表现出一些差异。因此，我们提议它们是玫瑰螺菌属内三个新物种的成员，即海洋玫瑰螺菌新种、纳瓦拉玫瑰螺菌新种和嗜硫代硫酸盐玫瑰螺菌新种，菌株CE2105、SE3104和AT2115分别作为模式菌株。因此，还给出了玫瑰螺菌属的修订描述。
