Duressa Dechassa, Soliman Khairy, Chen Dongquan
Department of Natural Resources and Environmental Sciences, Alabama A&M University, Normal, AL 35762, USA.
Int J Plant Genomics. 2010;2010. doi: 10.1155/2010/164862. Epub 2010 Oct 3.
Soybean is one of the most aluminum (Al) sensitive plants. The complex inheritance of Al tolerance trait has so far undermined breeding efforts to develop Al-tolerant soybeans. Discovering the genetic factors underlying the Al tolerance mechanisms would undoubtedly accelerate the pace of such endeavor. As a first step toward this goal, we analyzed the transcriptome profile in roots of Al-tolerant soybean line PI 416937 comparing Al-treated and untreated control plants using DNA microarrays. Many genes involved in transcription activation, stress response, cell metabolism and signaling were differentially expressed. Patterns of gene expression and mechanisms of Al toxicity and tolerance suggest that Cys2His2 and ADR6 transcription activators, cell wall modifying enzymes, and phytosulfokines growth factor play role in soybean Al tolerance. Our data provide insights into the molecular mechanisms of soybean Al tolerance and will have practical value in genetic improvement of Al tolerance trait.
大豆是对铝（Al）最为敏感的植物之一。迄今为止，耐铝性状复杂的遗传特性阻碍了培育耐铝大豆的育种工作。发现耐铝机制背后的遗传因素无疑将加快这一进程。作为朝着这一目标迈出的第一步，我们使用DNA微阵列分析了耐铝大豆品系PI 416937的根部转录组图谱，比较了铝处理和未处理的对照植株。许多参与转录激活、应激反应、细胞代谢和信号传导的基因存在差异表达。基因表达模式以及铝毒性和耐受性机制表明，Cys2His2和ADR6转录激活因子、细胞壁修饰酶以及植物磺肽素生长因子在大豆耐铝性中发挥作用。我们的数据为大豆耐铝的分子机制提供了见解，并且在耐铝性状的遗传改良方面具有实际价值。
