a CSIR - Indian Institute of Chemical Biology , Drug Development Diagnostics and Biotechnology Division , Kolkata , West Bengal , India.
b Department of Pharmaceutical Technology , Jadavpur University , Kolkata , West Bengal , India.
Pharm Biol. 2016 Nov;54(11):2536-2546. doi: 10.3109/13880209.2016.1168854. Epub 2016 May 25.
Termitomyces clypeatus (Lyophyllaceae) is a filamentous edible mushroom, having ethnomedicinal uses. However, information about the antioxidant, anticancer and antitumour properties of this mushroom remains to be elucidated.
The study examines the in vitro antioxidant, anticancer and in vivo antitumour activity of T. clypeatus.
Antioxidant activity was evaluated with seven in vitro assays. Cytotoxicity of T. clypeatus was tested against a panel of cancer cells lines including U373MG, MDA-MB-468, HepG2, HL-60, A549, U937, OAW-42 and Y-79 using MTT assay. The antitumour activity of aqueous extract was evaluated against Ehrlich ascites carcinoma (EAC) tumour model in Swiss albino mice.
HPLC analysis of aqueous extract revealed the presence of sugar entities. Termitomyces clypeatus showed excellent in vitro antioxidant activity. Termitomyces clypeatus was found cytotoxic against all cancer cells, among which it showed higher activity against U937 (IC 25 ± 1.02 μg/mL). Treatment of EAC-bearing mice with varied doses of aqueous extract significantly (p < 0.01) reduced tumour volume, viable tumour cell count and improved haemoglobin content, RBC count, mean survival time, tumour inhibition and % increase life span. The enhanced antioxidant status in treated animals was evident from the decline in the levels of lipid peroxidation, increased levels of glutathione, catalase and superoxide dismutase.
The analyzed data indicate that the aqueous extract of T. clypeatus exhibits significant antitumour activity, which might be due to the antioxidant effects on EAC bearing hosts.
Termitomyces clypeatus possesses anticancer activity, valuable for application in food and drug products.
齿菌（Lyophyllaceae）是一种丝状可食用蘑菇，具有民族医学用途。然而，关于这种蘑菇的抗氧化、抗癌和抗肿瘤特性的信息仍有待阐明。
本研究检查了齿菌的体外抗氧化、抗癌和体内抗肿瘤活性。
使用七种体外测定法评估抗氧化活性。使用 MTT 测定法测试齿菌对包括 U373MG、MDA-MB-468、HepG2、HL-60、A549、U937、OAW-42 和 Y-79 在内的一系列癌细胞系的细胞毒性。使用瑞士白化病小鼠的艾氏腹水癌（EAC）肿瘤模型评估水提取物的抗肿瘤活性。
HPLC 分析水提取物显示存在糖实体。齿菌表现出出色的体外抗氧化活性。齿菌对所有癌细胞均具有细胞毒性，其中对 U937 的活性更高（IC 25±1.02μg/mL）。用不同剂量的水提取物治疗 EAC 荷瘤小鼠可显著（p<0.01）降低肿瘤体积、存活肿瘤细胞计数并提高血红蛋白含量、红细胞计数、平均存活时间、肿瘤抑制率和%延长寿命。从治疗动物抗氧化状态的增强可以明显看出，脂质过氧化水平下降，谷胱甘肽、过氧化氢酶和超氧化物歧化酶水平升高。
分析数据表明，齿菌的水提取物具有显著的抗肿瘤活性，这可能是由于其对 EAC 荷瘤宿主的抗氧化作用。
齿菌具有抗癌活性，可用于食品和药物产品。
