体外初步研究酸枣仁对肿瘤细胞系的抑制作用及诱导凋亡作用。
Evaluation of inhibitory effect and apoptosis induction of Zyzyphus Jujube on tumor cell lines, an in vitro preliminary study.
机构信息
Biotechnology Department, Vaccine and Serum Research Institute, Mashhad, Iran,
出版信息
Cytotechnology. 2008 Feb;56(2):105-11. doi: 10.1007/s10616-008-9131-6. Epub 2008 Feb 9.
Abstract
In the present study, water extract of dried fruit of Zyzyphus Jujube was tested for its possible anticancer effect and induction of apoptosis on human tumor cell lines, HEp-2, HeLa and Jurkat cell lines. The inhibitory effect of water extract of this fruit on cell proliferation was assessed by MTT colorimetric assay. The induction of apoptosis of this extract was analyzed by DNA fragmentation analysis. Zyzyphus Jujube extract showed inhibitory effects on mentioned cell lines. Jurkat leukemic line was found the most sensitive cells with IC50 of 0.1 mug mL(-1). Our study also showed a typical DNA laddering in this cell line. The present study showed cytotoxic activity of Zyzyphus Jujube on tumor cells. Although Zyzyphus Jujube has useful compounds for medical applications.
摘要
在本研究中，测试了大枣干果果水提取物对人肿瘤细胞系 HEp-2、HeLa 和 Jurkat 细胞系的可能抗癌作用和诱导细胞凋亡作用。用 MTT 比色法评估这种水果水提取物对细胞增殖的抑制作用。通过 DNA 片段分析分析这种提取物诱导细胞凋亡的作用。大枣提取物对上述细胞系显示出抑制作用。Jurkat 白血病细胞系被发现是最敏感的细胞，IC50 为 0.1 μg mL(-1)。我们的研究还显示该细胞系出现典型的 DNA 梯状。本研究表明大枣对肿瘤细胞具有细胞毒性作用。尽管大枣含有用于医疗应用的有用化合物。
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