Halámek Jan, Bocharova Vera, Chinnapareddy Soujanya, Windmiller Joshua Ray, Strack Guinevere, Chuang Min-Chieh, Zhou Jian, Santhosh Padmanabhan, Ramirez Gabriela V, Arugula Mary A, Wang Joseph, Katz Evgeny
Department of Chemistry and Biomolecular Science, Clarkson University, Potsdam, NY 13699-5810, USA.
Mol Biosyst. 2010 Dec;6(12):2554-60. doi: 10.1039/c0mb00153h. Epub 2010 Oct 18.
A multi-enzyme biocatalytic cascade processing simultaneously five biomarkers characteristic of traumatic brain injury (TBI) and soft tissue injury (STI) was developed. The system operates as a digital biosensor based on concerted function of 8 Boolean AND logic gates, resulting in the decision about the physiological conditions based on the logic analysis of complex patterns of the biomarkers. The system represents the first example of a multi-step/multi-enzyme biosensor with the built-in logic for the analysis of complex combinations of biochemical inputs. The approach is based on recent advances in enzyme-based biocomputing systems and the present paper demonstrates the potential applicability of biocomputing for developing novel digital biosensor networks.
