Radziejewska Iwona, Borzym-Kluczyk Małgorzata, Kisiel Dariusz G, Namiot Zbigniew, Wosek Joanna, Gindzieński Andrzej
Department of Medical Chemistry, Medical University of Białystok, ul. Mickiewicza 2a, 15-879, Bialystok 8, Poland.
Dig Dis Sci. 2008 Oct;53(10):2641-5. doi: 10.1007/s10620-008-0224-z. Epub 2008 Apr 12.
Helicobacter pylori is considered as a causative agent of gastritis, duodenal and gastric ulcers, and gastric cancer. During inflammation, association of the pathogen of gastric epithelial cells and mucins is considered important. It was postulated that Lewis b structures of secretory MUC 5AC mucin can be a receptor for the bacterium. Some authors also suggest that epithelial MUC 1 mucin may be implicated in the mechanism of infection. The main aim of our work was to support this last suggestion by evaluation of the possible changes in MUC 1 and Lewis a and b levels in gastric juice before and at the end of eradication treatment. The gastric juices of ten examined patients were chromatographed on a Sepharose 4 B column, electrotransferred on Immobilon P membranes, and assessed for MUC 1 and Lewis a and b structures using monoclonal antibodies. In 90% of examined patients, higher amounts of MUC 1 mucin were observed at the end of eradication treatment. Similar results for Lewis a and b structures were found. In the case of MUC 1 and Lewis b, the differences were statistically significant. Helicobacter pylori influences expression of the soluble form of MUC 1 mucin and Lewis a and b structures present in gastric juice.
幽门螺杆菌被认为是胃炎、十二指肠溃疡、胃溃疡和胃癌的病原体。在炎症过程中，胃上皮细胞病原体与粘蛋白的结合被认为很重要。据推测，分泌型MUC 5AC粘蛋白的Lewis b结构可能是该细菌的受体。一些作者还表明，上皮MUC 1粘蛋白可能与感染机制有关。我们这项工作的主要目的是通过评估根除治疗前后胃液中MUC 1以及Lewis a和b水平的可能变化来支持这一最新观点。对10名受检患者的胃液在琼脂糖4 B柱上进行层析，电转移至Immobilon P膜上，并使用单克隆抗体评估MUC 1以及Lewis a和b结构。在90%的受检患者中，在根除治疗结束时观察到MUC 1粘蛋白含量更高。Lewis a和b结构也有类似结果。就MUC 1和Lewis b而言，差异具有统计学意义。幽门螺杆菌会影响胃液中可溶性MUC 1粘蛋白以及Lewis a和b结构的表达。
