Biodiversity Division, National Institute for Agro-Environmental Sciences, Tsukuba, Ibaraki 305-8604, Japan.
Nat Prod Res. 2010 Oct;24(17):1637-42. doi: 10.1080/14786419.2010.482050.
The ¹³C labels of [¹³C]carbon dioxide and D-[¹³C₆]glucose were incorporated into cyanamide (NH₂CN) when they were administered to Vicia villosa subsp. varia shoots. In contrast, the administration of sodium [2,3-¹³C₂]pyruvate did not affect the relative area of the [M + 1]+ ion of cyanamide in the gas chromatography-mass spectrometry analysis. [2,3-¹³C₂]pyruvate was incorporated into organic acids that are part of the citric acid cycle, such as succinate and fumarate, confirming that the shoots absorbed and metabolised it. These observations demonstrated that the carbon atom of cyanamide is derived from any of the carbohydrates that are present upstream of pyruvate in the metabolic pathway.
当 administered 到 Vicia villosa subsp. varia shoots 时，[¹³C]carbon dioxide 和 D-[¹³C₆]glucose 的¹³C 标记被结合到氰胺 (NH₂CN) 中。相比之下，施用 sodium [2,3-¹³C₂]pyruvate 不会影响气相色谱-质谱分析中氰胺[M+1]+离子的相对面积。[2,3-¹³C₂]pyruvate 被结合到柠檬酸循环中的有机酸盐，如琥珀酸和延胡索酸，证实了 shoots 吸收和代谢了它。这些观察结果表明，氰胺的碳原子来自于代谢途径中丙酮酸上游存在的任何碳水化合物。
