Kamo Tsunashi, Kato Kenji, Hiradate Syuntaro, Nakajima Eri, Fujii Yoshiharu, Hirota Mitsuru
Department of Bioscience and Biotechnology, Faculty of Agriculture, Shinshu University, 8304 Minami-minowa, Kami-ina, Nagano 399-4598, Japan.
Nat Prod Res. 2006 May 10;20(5):429-33. doi: 10.1080/14786410500143583.
Cyanamide (NH(2)CN) has recently been isolated as a plant growth inhibitor from Vicia villosa, which is the first discovery of cyanamide from natural sources. To reveal the presence of the biosynthesized cyanamide in plants, 3.4 mM potassium ((15)N)nitrate was administered to 15- to 35-day-old plants of V. villosa, from which the cyanamide was purified and subjected to GC/MS analysis. The isotopic ratio (15)N/((14)N + (15)N) of the cyanamide was calculated to be 0.143, while that of the cyanamide extracted from V. villosa grown in the presence of a natural N source was 0.0065. The (15)N-enrichment proved de novo biosynthesis of cyanamide.
氰胺（NH(2)CN）最近从野豌豆中被分离出来，作为一种植物生长抑制剂，这是首次从天然来源发现氰胺。为了揭示植物中生物合成的氰胺的存在，向15至35日龄的野豌豆植株施用3.4 mM硝酸钾（(15)N），从中纯化氰胺并进行气相色谱/质谱分析。计算得出氰胺的同位素比(15)N/((14)N + (15)N)为0.143，而从在天然氮源存在下生长的野豌豆中提取的氰胺的同位素比为0.0065。(15)N富集证明了氰胺的从头生物合成。
