Kamo Tsunashi, Hiradate Syuntaro, Fujii Yoshiharu
Department of Biological Safety, National Institute for Agro-Environmental Sciences, 3-1-3 Kan-nondai, Tsukuba, Ibaraki 305-8604, Japan.
J Chem Ecol. 2003 Feb;29(2):275-83. doi: 10.1023/a:1022621709486.
Cyanamide was isolated from the leaves and stems of hairy vetch (Vicia villosa), guided by plant growth inhibitory activity against lettuce (Lectuca sativa) seedlings. A large proportion of the inhibitory activity in the crude extract was explained by the presence of cyanamide, suggesting it to be a possible allelochemical in this species. The amount in a 9-day-old seedling, which had been grown without nutrients, reached approx. 40 times that of a nongerminated seed, demonstrating cyanamide biosynthesis in the seedlings. This is the first report on the isolation of a possible allelochemical from hairy vetch and also of the finding of cyanamide as a natural product.
在对生菜（Lactuca sativa）幼苗具有植物生长抑制活性的引导下，从毛苕子（Vicia villosa）的叶和茎中分离出了氰胺。粗提物中很大一部分抑制活性可归因于氰胺的存在，这表明氰胺可能是该物种中的一种化感物质。在无营养条件下生长9天的幼苗中氰胺的含量，达到了未发芽种子的约40倍，这证明了幼苗中氰胺的生物合成。这是关于从毛苕子中分离出一种可能的化感物质以及发现氰胺作为天然产物的首次报道。
