Du Ran, Li Shizhong, Zhang Xiaoqing, Wang Li
Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing 100084, China.
Sheng Wu Gong Cheng Xue Bao. 2010 Jul;26(7):960-5.
The recalcitrance of lignocellulosic biomass makes its hydrolysis by cellulases less effective, and the consolidated bioprocessing (CBP) strategy that combines enzyme production, cellulose hydrolysis and fermentation, particularly the synergetic role of different microbes in attacking cellulose component could be a solution. In this article, a facultative anaerobe microbial consortium named H was isolated, which exhibited high stability even after 30 subcultures, with pH ranging from 6 to 9. Within three days, 0.5 g filter paper immerged in 100 mL PCS buffer was completely degraded, and 1.54 g/L ethanol was produced, correspondingly. Further analysis on the component of the microbe consortium was carried out though 16S rDNA and DGGE, and Clostridium thermosuccinogene, Clostridium straminisolvens and Clostridium isatidis that can directly convert cellulose to ethanol were identified, indicating that Clostridium spp. played important role in cellulose degradation through the synergistic coordination of different species, and the characterization of the consortium will benefit the analysis of the underlying mechanisms as well as the optimization of the CBP process for more efficient cellulose degradation and ethanol production.
木质纤维素生物质的顽固性使得纤维素酶对其水解效果不佳，而将酶生产、纤维素水解和发酵相结合的固态发酵（CBP）策略，特别是不同微生物在攻击纤维素成分中的协同作用可能是一种解决方案。在本文中，分离出了一种名为H的兼性厌氧微生物群落，即使经过30次传代培养，该群落仍表现出高稳定性，pH范围为6至9。在三天内，浸入100 mL PCS缓冲液中的0.5 g滤纸被完全降解，相应地产生了1.54 g/L乙醇。通过16S rDNA和DGGE对微生物群落的成分进行了进一步分析，鉴定出了可直接将纤维素转化为乙醇的嗜热琥珀酸梭菌、解纤维梭菌和菘蓝梭菌，这表明梭菌属通过不同物种的协同作用在纤维素降解中发挥了重要作用，该群落的表征将有助于分析潜在机制以及优化CBP工艺，以实现更高效的纤维素降解和乙醇生产。
