Food Biotechnology Division, National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki 305-8642, Japan.
Fungal Biol. 2013 Mar;117(3):220-6. doi: 10.1016/j.funbio.2013.02.002. Epub 2013 Feb 20.
Ethanol production by Flammulina velutipes from high substrate concentrations was evaluated. F. velutipes produces approximately 40-60 g l(-1) ethanol from 15% (w/v) D-glucose, D-fructose, D-mannose, sucrose, maltose, and cellobiose, with the highest conversion rate of 83% observed using cellobiose as a carbon source. We also attempted to assess direct ethanol fermentation from sugarcane bagasse cellulose (SCBC) by F. velutipes. The hydrolysis rate of 15% (w/v) SCBC with commercial cellulase was approximately 20%. In contrast, F. velutipes was able to produce a significant amount of ethanol from 15% SCBC with the production of β-glucosidase, cellobohydrolase, and cellulase, although the addition of a small amount of commercial cellulase to the culture was required for the conversion. When 9 mg g(-1) biomass of commercial cellulase was added to cultures, 0.36 g of ethanol was produced from 1 g of cellulose, corresponding to an ethanol conversion rate of 69.6%. These results indicate that F. velutipes would be useful for consolidated bioprocessing of lignocellulosic biomass to bioethanol.
评估了金针菇从高底物浓度生产乙醇的情况。金针菇从 15%（w/v）的 D-葡萄糖、D-果糖、D-甘露糖、蔗糖、麦芽糖和纤维二糖中可生产约 40-60 g l(-1)的乙醇，以纤维二糖作为碳源时观察到的最高转化率为 83%。我们还尝试评估金针菇从甘蔗渣纤维素（SCBC）进行直接乙醇发酵。用商业纤维素酶水解 15%（w/v）的 SCBC 时，水解率约为 20%。相比之下，金针菇能够在产生β-葡萄糖苷酶、纤维二糖水解酶和纤维素酶的情况下从 15%的 SCBC 中生产大量的乙醇，尽管需要在培养物中添加少量的商业纤维素酶进行转化。当向培养物中添加 9 mg g(-1)生物质的商业纤维素酶时，从 1 g 纤维素中产生了 0.36 g 乙醇，对应的乙醇转化率为 69.6%。这些结果表明，金针菇可用于木质纤维素生物质的整合生物加工生产生物乙醇。
