Laboratory of Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, Yamaguchi, Japan.
Vector Borne Zoonotic Dis. 2011 May;11(5):515-21. doi: 10.1089/vbz.2010.0138. Epub 2010 Oct 18.
The natural infection of sand flies by Leishmania species was studied in the Andean areas of Peru where cutaneous leishmaniasis caused by Leishmania (Viannia) peruviana is endemic. Sand flies were captured by human bait and Center for Disease Control (CDC) light trap catches at Nambuque and Padregual, Department of La Libertad, Peru, and morphologically identified. Among 377 female sand flies dissected, the two dominant man-biting species were Lutzomyia (Helcocyrtomyia) peruensis (211 flies) and Lutzomyia (Helcocyrtomyia) caballeroi (151 flies). Another sand fly species captured by light trap was Warileya phlebotomanica (15 flies). The natural infection of sand flies by flagellates was detected in 1.4% of Lu. (H.) peruensis and 2.6% of Lu. (H.) caballeroi, and the parasite species were identified as Le. (V.) peruviana and Trypanosoma avium, respectively, by molecular biological methods. The results indicated that the vector species responsible for the transmission of leishmaniasis in the study areas is Lu. (H.) peruensis. In addition, the presence of Trypanosoma in man-biting sand fly species means that more careful consideration is necessary for vector research in areas of Andean Peru where leishmaniasis is endemic.
本研究调查了秘鲁安第斯地区沙蝇感染利什曼原虫的情况，该地区是利什曼原虫（Viannia）引起的皮肤利什曼病的流行区。在秘鲁拉利伯塔德省的南布克和帕德雷瓜尔，通过人诱饵和疾病控制中心（CDC）诱捕器捕获了沙蝇，并进行了形态学鉴定。在解剖的 377 只雌性沙蝇中，两种主要的人咬蚊种为 Lutzomyia（Helcocyrtomyia）peruensis（211 只）和 Lutzomyia（Helcocyrtomyia）caballeroi（151 只）。另一种通过诱捕器捕获的沙蝇物种为 Warileya phlebotomanica（15 只）。通过分子生物学方法检测到 1.4%的 Lu.（H.）peruensis 和 2.6%的 Lu.（H.）caballeroi 感染了鞭毛原生动物，鉴定出的寄生虫种分别为 Le.（V.）peruviana 和 Trypanosoma avium。结果表明，在研究地区传播利什曼病的媒介物种是 Lu.（H.）peruensis。此外，在人咬蚊种中发现了锥虫，这意味着在秘鲁安第斯地区的利什曼病流行区进行媒介研究时需要更加谨慎。
