School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong SAR, China.
Chin Med. 2010 Oct 18;5:35. doi: 10.1186/1749-8546-5-35.
Stroke caused by brain ischemia is the third leading cause of adult disability. Active prevention and early treatment of stroke targeting the causes and risk factors may decrease its incidence, mortality and subsequent disability. Pien Tze Huang (PZH), a Chinese medicine formula, was found to have anti-edema, anti-inflammatory and anti-thrombotic effects that can prevent brain damage. This study aims to investigate the potential mechanisms of the preventive effects of Pien Tze Huang on brain damage caused by chronic ischemia and hypertensive stroke in rats.
The effects of Pien Tze Huang on brain protein expression in spontaneously hypertensive rat (SHR) and stroke prone SHR (SHRsp) were studied with 2-D gel electrophoresis and mass spectrometric analysis with a matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)/TOF tandem mass spectrometer and on brain cell death with enzyme link immunosorbent assay (ELISA) and immunostaining.
Pien Tze Huang decreased cell death in hippocampus and cerebellum caused by chronic ischemia and hypertensive stroke. Immunostaining of caspase-3 results indicated that Pien Tze Huang prevents brain cells from apoptosis caused by ischemia. Brain protein expression results suggested that Pien Tze Huang downregulated QCR2 in the electron transfer chain of mitochondria preventing reactive oxygen species (ROS) damage and possibly subsequent cell death (caspase 3 assay) as caused by chronic ischemia or hypertensive stroke to hippocampus and cerebellum.
Pien Tze Huang showed preventive effects on limiting the damage or injury caused by chronic ischemia and hypertensive stroke in rats. The effect of Pien Tze Huang was possibly related to prevention of cell death from apoptosis or ROS/oxidative damage in mitochondria.
脑缺血引起的中风是导致成年人残疾的第三大原因。针对病因和危险因素积极预防和早期治疗中风，可以降低其发病率、死亡率和随后的残疾率。中药配方片仔癀具有消肿、抗炎和抗血栓作用，可预防脑损伤。本研究旨在探讨片仔癀预防慢性缺血和高血压性中风大鼠脑损伤的潜在机制。
采用二维凝胶电泳和基质辅助激光解吸/电离飞行时间（MALDI-TOF）/TOF 串联质谱技术，结合酶联免疫吸附试验（ELISA）和免疫染色，研究片仔癀对自发性高血压大鼠（SHR）和中风易感 SHR（SHRsp）脑蛋白表达的影响。
片仔癀降低了慢性缺血和高血压性中风引起的海马和小脑细胞死亡。Caspase-3 免疫染色结果表明，片仔癀可预防缺血引起的脑细胞凋亡。脑蛋白表达结果表明，片仔癀下调了线粒体电子传递链中的 QCR2，从而防止活性氧（ROS）损伤和随后的细胞死亡（Caspase 3 检测），这是由慢性缺血或高血压性中风对海马和小脑造成的。
片仔癀对限制慢性缺血和高血压性中风大鼠的损伤具有预防作用。片仔癀的作用可能与预防细胞凋亡或线粒体中 ROS/氧化损伤引起的细胞死亡有关。
