Mondranondra I O, Che C T, Rimando A M, Vajrodaya S, Fong H H, Farnsworth N R
College of Pharmacy, University of Illinois, Chicago 60680.
Pharm Res. 1990 Dec;7(12):1269-72. doi: 10.1023/a:1015937921880.
The pentane and CHCl3 fractions of a crude extract of Michelia floribunda exhibited cytotoxic activity when tested in KB and P388 tumor cell cultures. Repeated chromatography led to the isolation of three cytotoxic sesquiterpene lactones (costunolide, parthenolide, and santamarine) and a cytotoxic isoquinoline alkaloid (liriodenine). Inactive sesquiterpene lactones obtained during the course of this study included dihydroparthenolide and two new glucosides of dihydrotamaulipin A and dihydroreynosin (1 and 2). The structures of these new compounds were determined through interpretation of their spectroscopic data including 2D-NMR spectroscopy. Syringin was also isolated from the extract.
黄兰粗提物的戊烷和氯仿馏分在KB和P388肿瘤细胞培养物中进行测试时表现出细胞毒性活性。反复色谱分离得到了三种具有细胞毒性的倍半萜内酯（木香烯内酯、小白菊内酯和圣玛丽内酯）和一种具有细胞毒性的异喹啉生物碱（鹅掌楸碱）。在本研究过程中获得的无活性倍半萜内酯包括二氢小白菊内酯以及二氢塔毛利平A和二氢雷诺辛的两种新糖苷（1和2）。这些新化合物的结构通过对其光谱数据（包括二维核磁共振光谱）的解释得以确定。还从提取物中分离出了紫丁香苷。
