Thorman Royne, Lundahl Joachim, Yucel-Lindberg Tülay, Hylander Britta
Department of Dental Medicine, Public Dental Service, Karolinska University Hospital, Karolinska Institutet, Solna, Sweden.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Nov;110(5):597-604. doi: 10.1016/j.tripleo.2010.07.007.
The aim of this study was to evaluate correlations between levels of cytokines in secreted stimulated saliva in patients with chronic kidney disease (CKD) and hyposalivation.
Seventy patients with clearance <20 mL/min/1.73 m(2) were evaluated; 40 were predialysis, 21 hemodialysis, and 9 peritoneal dialysis, and they were matched with 70 control subjects. Salivary flow rate was measured and submandibular/sublingual saliva collected. Analyses were performed for whole protein content using a protein assay, and levels of tumor necrosis factor (TNF) α, interleukin (IL) 1β, γ-interferon (γ-INF), IL-6, IL-8, IL-10, monocyte chemotactic protein (MCP) 1, and soluble intercellular adhesion molecule (sICAM) 1, by using Luminex technology.
Patients with CKD had lower (P = .03) stimulated salivary secretion rate and higher salivary whole protein concentration (P = .002) than control subjects. Concentrations of IL-8 (P = .03) and MCP-1 (P = .002) were decreased and TNF-α/IL-10 (P = .05) and IL-8/IL10 (P = .03) ratios were decreased in CKD patients. CKD patients with low secretion levels of stimulated saliva expressed decreased levels of TNF-α (P = .04), IL-1β (P = .02), γ-INF (P = .03), IL-6 (P = .003), IL-8 (P = .005), MCP-1 (P = .006), and sICAM-1 (P = .02).
Salivary cytokines and secretion rates are significantly decreased in CKD patients. Further research is necessary to understand operating mechanisms and clinical implications of the down-regulation of inflammatory markers in saliva.
本研究旨在评估慢性肾脏病（CKD）患者分泌性刺激唾液中细胞因子水平与唾液分泌减少之间的相关性。
对70名肌酐清除率<20 mL/分钟/1.73 m²的患者进行评估；其中40名处于透析前阶段，21名接受血液透析，9名接受腹膜透析，他们与70名对照受试者进行匹配。测量唾液流速并收集颌下/舌下唾液。使用蛋白质测定法分析全蛋白含量，并采用Luminex技术检测肿瘤坏死因子（TNF）α、白细胞介素（IL）1β、γ干扰素（γ-INF）、IL-6、IL-8、IL-10、单核细胞趋化蛋白（MCP）1和可溶性细胞间黏附分子（sICAM）1的水平。
与对照受试者相比，CKD患者的刺激唾液分泌率较低（P = 0.03），唾液全蛋白浓度较高（P = 0.002）。CKD患者中IL-8（P = 0.03）和MCP-1（P = 0.002）的浓度降低，TNF-α/IL-10（P = 0.05）和IL-8/IL10（P = 0.03）的比值降低。刺激唾液分泌水平低的CKD患者TNF-α（P = 0.04）、IL-1β（P = 0.02）、γ-INF（P = 0.03）、IL-6（P = 0.003）、IL-8（P = 0.005）、MCP-1（P = 0.006）和sICAM-1（P = 0.02）的水平降低。
CKD患者唾液中的细胞因子和分泌率显著降低。有必要进一步研究以了解唾液中炎症标志物下调的作用机制和临床意义。
