通过彗星试验测量慢性肾病患者唾液腺组织中的DNA损伤。
DNA damage in salivary gland tissue in patients with chronic kidney disease, measured by the comet assay.
作者信息
Ersson Clara, Thorman Royne, Rodhe Ylva, Möller Lennart, Hylander Britta
机构信息
Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.
出版信息
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Aug;112(2):209-15. doi: 10.1016/j.tripleo.2011.03.016.
OBJECTIVE
The aim of this observational study was to investigate the relationship between DNA damage in minor accessory salivary glands, hyposalivation, and inflammation in patients with chronic kidney disease (CKD).
STUDY DESIGN
DNA strand breaks and oxidative DNA lesions in salivary glands, inflammatory markers, and uremic state were measured in 79 patients with CKD and matched controls.
RESULTS
CKD patients not yet on dialysis had significantly more, and dialysis patients significantly less, DNA strand breaks in salivary tissue compared with controls. All measured inflammatory markers were higher in patients with CKD compared with controls. Salivary secretion rates were significantly lower in dialysis patients compared with controls. A high level of salivary secretion rate at rest significantly predicted a high level of DNA strand breaks in patients with CKD.
CONCLUSIONS
Dialysis patients had fewer DNA strand breaks in minor accessory salivary glands than controls, suggesting that peripheral tissue is differently affected by CKD than leukocytes.
目的
本观察性研究旨在探讨慢性肾脏病（CKD）患者小唾液腺中的DNA损伤、唾液分泌减少与炎症之间的关系。
研究设计
对79例CKD患者及其匹配的对照组测量唾液腺中的DNA链断裂和氧化性DNA损伤、炎症标志物及尿毒症状态。
结果
与对照组相比，尚未接受透析的CKD患者唾液组织中的DNA链断裂明显更多，而透析患者则明显更少。与对照组相比，CKD患者所有测量的炎症标志物均更高。与对照组相比，透析患者的唾液分泌率明显更低。静息时高水平的唾液分泌率显著预示着CKD患者高水平的DNA链断裂。
结论
透析患者小唾液腺中的DNA链断裂比对照组少，这表明CKD对周围组织的影响与白细胞不同。
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