Jampel H D
Glaucoma Service, Wilmer Ophthalmological Institute, Johns Hopkins University School of Medicine, Baltimore, Md. 21205.
Arch Ophthalmol. 1990 Sep;108(9):1323-5. doi: 10.1001/archopht.1990.01070110139038.
Successful glaucoma filtration surgery depends on the incomplete healing of the surgical wound, with formation of a filtration bleb. In most other tissues, however, complete healing is the rule. I have explored the possibility that the high concentration of ascorbic acid normally present in aqueous humor inhibits wound healing after filtration surgery. At the concentration normally present in aqueous humor (1.1 mmol/L), ascorbic acid decreased the plating efficiency of cell suspensions of human Tenon's capsule fibroblasts by a mean (+/- SD) of 40% +/- 10%. When added to low-density monolayer cultures of fibroblasts, ascorbic acid decreased the cell number by 90% +/- 5%, an effect that was completely prevented by catalase. When added to confluent cultures, the cell number was decreased by only 14% +/- 2%. If ascorbic acid has similar effects on fibroblasts in vivo, it may contribute to the incomplete wound healing that characterizes successful glaucoma surgery.
成功的青光眼滤过手术依赖于手术伤口的不完全愈合以及滤过泡的形成。然而，在大多数其他组织中，完全愈合才是常态。我探讨了房水中通常存在的高浓度抗坏血酸抑制滤过手术后伤口愈合的可能性。在房水正常存在的浓度（1.1 mmol/L）下，抗坏血酸使人类眼球筋膜成纤维细胞悬浮液的接种效率平均（±标准差）降低了40%±10%。当添加到成纤维细胞的低密度单层培养物中时，抗坏血酸使细胞数量减少了90%±5%，而过氧化氢酶可完全阻止这种效应。当添加到汇合培养物中时，细胞数量仅减少了14%±2%。如果抗坏血酸在体内对成纤维细胞有类似作用，它可能有助于成功青光眼手术所特有的不完全伤口愈合。
