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土耳其黑海地区野生食用蘑菇的抗氧化、抗菌活性及化学成分筛选。

Screening of antioxidant, antimicrobial activities and chemical contents of edible mushrooms wildly grown in the black sea region of Turkey.

作者信息

Ozen Tevfik, Darcan Cihan, Aktop Oznur, Turkekul Ibrahim

机构信息

Department of Chemistry, Faculty of Art and Sciences, Ondokuz Mayis University, 55139-Samsun, Turky. Turkey.

出版信息

Comb Chem High Throughput Screen. 2011 Feb;14(2):72-84. doi: 10.2174/138620711794474079.

Abstract

The antioxidative activity of the methanol extracts obtained from twelve commonly consumed wild edible mushrooms was investigated according to the phosphomolybdenum method, inhibition of linoleic acid peroxidation, reducing power, metal chelating, O(2)(˙-), DPPH(˙), peroxide and H(2)O(2) scavenging activity in the Black Sea Region of Turky, and compared to standard antioxidant compounds such as a-tocopherol, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), and trolox. Total phenolic compounds, flavonoids, anthocyanins, ascorbic acid, β-carotene and lycopene in the extracts were measured. The antimicrobial activities of extracts were also evalated against gram (-) and (+) bacteria and yeast with disc diffusion method. Hydnum repandum, Lactarius delicious, Lactarius camphoratus, Lactarius piperatus, Ramaria flava, and Agaricus bisporus were greater than α-tocopherol and BHT on antioxidant activity. The lipid peroxidation of L. camphoratus and Lactarius volemus was excellent, and was 58.05% and 61.44% respectively. The DPPH(˙) scavenging effects decreased in the order of R. flava>BHT> Macrolepiota procera>L. camphoratus>L. piperatus>Lactarius volemus>a-tocopherol and were, at 500 µg/ml and were 80.38, 76.61, 69.74, 61.55, 61.47, 61.09, 60.65%, respectively. All the extracts of mushroom also had the stronger chelating effect according to standards. The O(2)(˙-) scavenging effect of extracts of the mushroom species was in order of R. flava>Boletus edulis>Leatiporus sulphureus>M. procera>standards. L. piperatus, L. camphorates, L. volemus, A. bisporus, Chanterellus cibarius, L. sulphureus, H. repandum showed strong antimicrobial activity, especially on E. coli. H. repandum exhibited to be more effective on P. aeruginosa than other bacterial strains.

摘要

采用磷钼酸法、亚油酸过氧化抑制法、还原力法、金属螯合能力法、超氧阴离子自由基(O₂˙⁻)清除法、1,1-二苯基-2-三硝基苯肼自由基(DPPH˙)清除法、过氧化物清除法和过氧化氢(H₂O₂)清除法,对土耳其黑海地区12种常见野生可食用蘑菇的甲醇提取物的抗氧化活性进行了研究,并与α-生育酚、丁基羟基茴香醚(BHA)、二丁基羟基甲苯(BHT)和特洛克斯等标准抗氧化化合物进行了比较。测定了提取物中的总酚类化合物、黄酮类化合物、花青素、抗坏血酸、β-胡萝卜素和番茄红素含量。还采用纸片扩散法评估了提取物对革兰氏阴性菌、革兰氏阳性菌和酵母菌的抗菌活性。齿菌、美味乳菇、樟脑乳菇、辣乳菇、黄枝瑚菌和双孢蘑菇的抗氧化活性高于α-生育酚和BHT。樟脑乳菇和绒毛乳菇的脂质过氧化抑制效果极佳,分别为58.05%和61.44%。黄枝瑚菌、BHT、高大环柄菇、樟脑乳菇、辣乳菇、绒毛乳菇、α-生育酚的DPPH˙清除效果依次降低,在500μg/ml时分别为80.38%、76.61%、69.74%、61.55%、61.47%、61.09%、60.65%。与标准物相比,所有蘑菇提取物也具有更强的螯合能力。蘑菇提取物的O₂˙⁻清除效果依次为黄枝瑚菌>美味牛肝菌>硫黄多孔菌>高大环柄菇>标准物。辣乳菇、樟脑乳菇、绒毛乳菇、双孢蘑菇、鸡油菌、硫黄多孔菌、齿菌表现出较强的抗菌活性,尤其是对大肠杆菌。齿菌对铜绿假单胞菌的抗菌效果比其他菌株更显著。

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