Lesnik F, Chudý D, Bogdan J, Vrtiak O J, Rudic M
Vet Med (Praha). 1978 Jul;23(7):421-30.
An experiment was performed to study the immunogenicity of the dermal antigen of Marek's disease virus, extracted from the skin of 30-day-old chickens, infected with Marek's disease virus on the first day of life. Three kinds of samples were tested: (1) dermal antigen centrifuged at 10 000 g per 0.5 h, (2) dermal antigen centrifugated at 10 000 g per 0.5 h and 100 000 g per 1 h, (3) dermal antigen treated like sample (2) and partly purified by DEAE-cellulose chromatography. Samples (1) and (2) were inoculated to two-day-old chickens and the vaccination was repeated, using complete Freund's adjuvant, 21 days later. Sample (3) was inoculated to two-day-old chickens with DEAE-dextran. All the three groups were challenged together with the controls (non-vaccinated chickens) on the seventh day after the first vaccination. A reduction of mortality was observed in the chickens vaccinated with and re-vaccinated with sample (1) (23.07%) and in the chickens vaccinated with sample (3) (30.76%). The chickens of the latter group were the last to start dying from Marek's disease--only after the 10th week of life. In the chickens which had been vaccinated and revaccinated with sample (2) the mortality was not reduced. The study is continued, with particular emphasis on the relationship of DEAE-dextran to protection against Marek's disease.
进行了一项实验，以研究从出生第一天就感染马立克氏病病毒的30日龄鸡的皮肤中提取的马立克氏病病毒皮肤抗原的免疫原性。测试了三种样品：（1）每0.5小时以10000g离心的皮肤抗原，（2）每0.5小时以10000g和每1小时以100000g离心的皮肤抗原，（3）像样品（2）一样处理并通过DEAE-纤维素色谱法部分纯化的皮肤抗原。将样品（1）和（2）接种到2日龄鸡中，并在21天后使用完全弗氏佐剂重复接种。将样品（3）与DEAE-葡聚糖一起接种到2日龄鸡中。在首次接种后的第7天，将所有三组与对照组（未接种疫苗的鸡）一起进行攻毒。在用样品（1）接种并再次接种的鸡中（23.07%）以及在用样品（3）接种的鸡中（30.76%）观察到死亡率降低。后一组鸡是最后开始死于马立克氏病的——仅在生命的第10周之后。在用样品（2）接种并再次接种的鸡中，死亡率没有降低。该研究仍在继续，特别强调DEAE-葡聚糖与预防马立克氏病的关系。
