Chen Ya-Hong, Zhang Ya-Nan, Tian Feng-Shou
Department of Chemistry, Zhoukou Normal University, Zhoukou, 466001, China; Department of Chemistry, Zhengzhou University, Zhengzhou, 450001, China.
Luminescence. 2015 May;30(3):263-8. doi: 10.1002/bio.2723. Epub 2014 Jun 13.
A new method for the determination of selenium based on its fluorescence quenching on the hemoglobin-catalyzed reaction of H2 O2 and l-tyrosine has been established. The effect of pH, foreign ions and the optimization of variables on the determination of selenium was examined. The calibration curve was found to be linear between the fluorescence quenching (F0 /F) and the concentration of selenium within the range of 0.16-4.00 µg/mL. The detection limit was 1.96 ng/mL and the relative standard deviation was 3.14%. This method can be used for the determination of selenium in Se-enriched garlic bulbs with satisfactory results.
基于硒对血红蛋白催化的H2O2与L-酪氨酸反应的荧光猝灭作用，建立了一种测定硒的新方法。考察了pH值、外来离子的影响以及测定硒时变量的优化。发现荧光猝灭（F0/F）与硒浓度在0.16 - 4.00 µg/mL范围内呈线性校准曲线。检测限为1.96 ng/mL，相对标准偏差为3.14%。该方法可用于富硒大蒜中硒的测定，结果令人满意。
