Department of Biomedical Engineering, Boston University, 44 Cummington Street, Fourth Floor, Boston, Massachusetts 02215, United States.
Nano Lett. 2010 Nov 10;10(11):4727-31. doi: 10.1021/nl103210p.
Rapid, chip-scale, and cost-effective single particle detection of biological agents is of great importance to human health and national security. We report real-time, high-throughput detection and sizing of individual, low-index polystyrene nanoparticles and H1N1 virus. Our widefield, common path interferometer detects nanoparticles and viruses over a very large sensing area, orders of magnitude larger than competing techniques. We demonstrate nanoparticle detection and sizing down to 70 nm in diameter. We clearly size discriminate nanoparticles with diameters of 70, 100, 150, and 200 nm. We also demonstrate detection and size characterization of hundreds of individual H1N1 viruses in a single experiment.
快速、芯片级、经济高效的生物制剂单颗粒检测对人类健康和国家安全至关重要。我们报告了对单个低折射率聚苯乙烯纳米颗粒和 H1N1 病毒的实时、高通量检测和尺寸分析。我们的宽场、共光路干涉仪在比竞争技术大几个数量级的超大传感面积上检测纳米颗粒和病毒。我们证明了直径低至 70nm 的纳米颗粒的检测和尺寸分析。我们可以清楚地区分直径为 70、100、150 和 200nm 的纳米颗粒。我们还在单个实验中演示了数百个单个 H1N1 病毒的检测和尺寸特征分析。
