Institute of Biology/Biophysics, Humboldt-University Berlin, Invalidenstrasse 42, 10115 Berlin, Germany.
J Am Chem Soc. 2010 Nov 17;132(45):16066-72. doi: 10.1021/ja105714r. Epub 2010 Oct 21.
Lipid domains in mammalian plasma membranes serve as platforms for specific recruitment or separation of proteins involved in various functions. Here, we have applied this natural strategy of lateral separation to functionalize lipid membranes at micrometer scale in a switchable and reversible manner. Membrane-anchored peptide nucleic acid and DNA, differing in their lipophilic moieties, partition into different lipid domains in model and biological membranes. Separation was visualized by hybridization with the respective complementary fluorescently labeled DNA strands. Upon heating, domains vanished, and both lipophilic nucleic acid structures intermixed with each other. Reformation of the lipid domains by cooling led again to separation of membrane-anchored nucleic acids. By linking appropriate structures/functions to complementary strands, this approach offers a reversible tool for triggering interactions among the structures and for the arrangement of reactions and signaling cascades on biomimetic surfaces.
哺乳动物质膜中的脂筏作为平台，用于特定招募或分离参与各种功能的蛋白质。在这里，我们应用这种天然的侧向分离策略，以可切换和可逆的方式在微米尺度上对脂质膜进行功能化。锚定在膜上的肽核酸和 DNA 因其亲脂部分的不同而在模型和生物膜中分配到不同的脂筏中。通过与各自互补的荧光标记 DNA 链杂交来可视化分离。加热时，脂筏消失，两种亲脂性核酸结构相互混合。通过冷却重新形成脂质筏，再次导致膜锚定核酸的分离。通过将适当的结构/功能与互补链连接，这种方法为触发结构之间的相互作用以及在仿生表面上安排反应和信号级联提供了一种可逆的工具。
