Department of Cardiothoracic Surgery, People's Hospital of Leshan, Leshan, Sichuan, 614000, People's Republic of China.
Anal Bioanal Chem. 2023 Mar;415(7):1305-1311. doi: 10.1007/s00216-022-04419-3. Epub 2022 Nov 12.
Cardiomyocyte-derived extracellular vesicles (EVs) are a promising class of biomarkers that can advance the diagnosis of many kinds of cardiovascular diseases. Herein, we develop a new electrochemical method for the feasible detection of cardiomyocyte-derived EVs in biological fluids. The core design of the method is the fabrication of a peptide-anchored biomimetic interface consisting of a lipid bilayer and peptide probes. On the one hand, the lipid bilayer provides excellent antifouling ability to the electrode interface and facilitates the anchoring of peptide probes. On the other hand, the peptide probes equip the electrode interface with excellent binding capability and affinity to CD172a, a specific marker of cardiomyocyte-derived EVs, thus enabling the efficient and selective detection of target EVs. Taking EVs derived from the heart myoblast cells H9C2 as the model target, the method displays a wide linear detection range from 1 × 10 to 1 × 10 particles/mL with a desirable detection limit of 132 particles/mL. Furthermore, the method shows good performance in biological fluids such as serum, and thus may have great potential for practical use in the diagnosis of cardiovascular diseases.
心肌细胞衍生的细胞外囊泡（EVs）是一类很有前途的生物标志物，可用于诊断多种心血管疾病。在此，我们开发了一种新的电化学方法，用于在生物体液中可行地检测心肌细胞衍生的 EVs。该方法的核心设计是构建由脂质双层和肽探针组成的肽锚定仿生界面。一方面，脂质双层为电极界面提供了出色的抗污能力，并有利于肽探针的锚定。另一方面，肽探针使电极界面具有与心肌细胞衍生 EVs 的特异性标志物 CD172a 优异的结合能力和亲和力，从而能够有效地选择性检测靶 EVs。以心肌细胞系 H9C2 衍生的 EVs 作为模型靶标，该方法在 1×10 到 1×10 个/mL 的宽线性检测范围内表现出良好的线性关系，检测限为 132 个/mL。此外，该方法在血清等生物体液中表现出良好的性能，因此在心血管疾病的诊断中具有很大的实际应用潜力。
