Faculty of Veterinary Science, Chulalongkorn University, Henri-Dunant Rd., Bangkok 10330, Thailand.
Vet Microbiol. 2011 Mar 24;148(2-4):413-8. doi: 10.1016/j.vetmic.2010.09.019. Epub 2010 Sep 29.
We investigated influenza interspecies transmission in two commercial swine farms in Thailand. Sera from swine-exposed workers (n=78), age-matched non-swine-exposed healthy people (n=60) and swine populations in both farms (n=85) were studied. Hemagglutination-inhibition (HI) assay was performed on Thai swine H1 viruses (swH1N1 and swH1N2) isolated from both farms. Thai human H1N1 (huH1N1) and pandemic H1N1 2009 (pH1N1) were also used as test antigens. The hemagglutinin (HA) 1 genes of swH1N1 and swH1N2 viruses were sequenced and shown to be genetically distinct from the Thai huH1N1 and pH1N1 viruses. Evidence of pig-to-human influenza virus transmission was found in farm workers with increased odds of elevated antibody titers to both swH1N1 (OR 42.63, 95% CI, 14.65-124) and swH1N2 (OR 58, 95% CI, 13.12-256.3) viruses. No evidence of human-to-pig influenza virus transmission was detected in this study.
我们在泰国的两家商业猪场调查了流感的种间传播。研究了接触猪的工人（n=78）、年龄匹配的未接触猪的健康人群（n=60）和两个猪场的猪群（n=85）的血清。对从两个猪场分离的泰国猪 H1 病毒（swH1N1 和 swH1N2）进行了血凝抑制（HI）试验。还使用了泰国人源 H1N1（huH1N1）和大流行 H1N1 2009（pH1N1）作为检测抗原。swH1N1 和 swH1N2 病毒的血凝素（HA）1 基因被测序，结果表明它们与泰国的 huH1N1 和 pH1N1 病毒在遗传上有明显区别。研究发现，接触猪的工人对 swH1N1（OR 42.63，95%CI，14.65-124）和 swH1N2（OR 58，95%CI，13.12-256.3）病毒的抗体滴度升高，这表明存在猪流感病毒向人类的传播。在这项研究中未发现人类流感病毒向猪的传播证据。
