Hu Yong, Ju Shao-Hua, Zhang Yin-Jie, Xiong Min, Xu Shi-Jun, Ma Yun-Tong, Zhong Zhen-Dong
Zhongguo Zhong Yao Za Zhi. 2014 May;39(10):1908-12.
To study the effect of Tongluo Xingnao effervescent tablets on learning and memory capacity and expression of Na(+)-K(+)-ATPase in hippocampus of rats with chronic cerebral ischemia-induced learning and memory dysfunction model.
The 2-VO method was used to establish sd rat model learning and memory dysfunction induced by chronic cerebral ischemia. The 50 rats in the successfully established model were randomly divided into the model control group, the Dihydroergotoxine Mesylate tablets group (0.7 mg x kg(-1), Tongluo Xingnao effervescent tablets high dose (7.56 g x kg(-1)), middle dose (3.78 g x kg(-1)) and low dose (1.59 g x kg(-1)) groups and the sham operation group (n = 10) as the control group. The groups were orally given 10 ml x kg(-1) x d(-1) drugs for consecutively 90 days. On the 86th day, Morris water maze was adopted for them. On the 90th day, a leaning and memory capacity test was held. The brain tissues were fixed with 10% formaldehyde and observed for pathomorphism after routine slide preparation and staining. The expression of hippocampal Na(+)-K(+)-ATPase was detected with immunohistochemistry and image quantitative analysis.
Compared with the model group, all of Tongluo Xingnao effervescent tablets groups showed significant decrease in the escape latency at the 5th day in the Morris water maze, and notable increase in the frequency of the first quadrant dwell, the frequency passing the escape platform and the frequency entering effective area (p < 0.05). According to the pathomorphological detection, the control group showed a significantly higher pathological score than the sham operation group (p < 0.01), the middle dose group showed a significantly lower pathological score than the model group (p < 0.05). According to the immunohistochemistical detection, the model control group showed a remarkably lower mean OD value of Na(+)-K(+)-ATPase than the sham operation group (p < 0.05), high and middle dose groups showed a significantly higher mean od value than the model control group (p < 0.01).
Tongluo Xingnao effervescent tablets can improve the learning and memory capacity, reduce pathological changes of hippocampal tissues of rats with chronic cerebral ischemia-induced learning and memory dysfunction model, and promote the expression of Na(+)-K(+)-ATPase in hippocampus.
研究通络醒脑泡腾片对慢性脑缺血致学习记忆功能障碍模型大鼠学习记忆能力及海马组织中钠钾ATP酶（Na(+)-K(+)-ATPase）表达的影响。
采用双侧颈总动脉永久结扎法（2-VO法）建立慢性脑缺血致SD大鼠学习记忆功能障碍模型。将成功造模的50只大鼠随机分为模型对照组、甲磺酸二氢麦角碱片组（0.7mg·kg(-1)）、通络醒脑泡腾片高剂量组（7.56g·kg(-1)）、中剂量组（3.78g·kg(-1)）、低剂量组（1.59g·kg(-1)）和假手术组（n = 10）作为对照组。各组均按10ml·kg(-1)·d(-1)灌胃给药，连续90天。于第86天进行Morris水迷宫实验，第90天进行学习记忆能力测试。取脑组织用10%甲醛固定，常规制片、染色后观察病理形态。采用免疫组织化学法及图像定量分析检测海马组织中Na(+)-K(+)-ATPase的表达。
与模型组比较，各通络醒脑泡腾片组在Morris水迷宫实验第5天逃避潜伏期均显著缩短，在第一象限停留次数、穿越平台次数及进入有效区域次数均显著增加（p < 0.05）。病理形态学检测结果显示，模型对照组病理评分显著高于假手术组（p < 0.01），通络醒脑泡腾片中剂量组病理评分显著低于模型组（p < 0.05）。免疫组织化学检测结果显示，模型对照组海马组织中Na(+)-K(+)-ATPase平均光密度值显著低于假手术组（p < 0.05），高、中剂量组平均光密度值显著高于模型对照组（p < 0.01）。
通络醒脑泡腾片可改善慢性脑缺血致学习记忆功能障碍模型大鼠的学习记忆能力，减轻海马组织病理改变，促进海马组织中Na(+)-K(+)-ATPase的表达。
