Huagn Yue, Xu Yu-ming, Zhang Jie-wen, Ren Xiu-hua, Suo Ai-qin
Department of Neurology, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2010 Oct;30(10):2408-10.
To examine the effect of estrogen on the expressions of phosphorylated Tau (P-Tau), ChAT and nerve growth factor (NGF) protein in the brain tissue of rat models of Alzheimer disease (AD).
Rat models of AD were established by injecting Aβ1-42 protein fragments in the right lateral ventricle. Two weeks later, 17β-estradiol tablets were implanted subcutaneously at the neck of the rats and maintained for 30 days. The pathological changes in the rats' brain neurons and alterations in the expressions of P-Tau, ChAT and NGF proteins were observed using HE staining and immunohistochemistry, respectively.
In the AD rats, neurofibrillary tangles occurred in the brain tissue, and estrogen treatment significantly reduced the formation of neurofibrillary tangles. Estrogen treatment also resulted in lowered P-Tau expression and increased ChAT and NGF protein expressions in comparison with those in the AD model rats.
Estrogen can up-regulate ChAT and NGF and down-regulate tau protein expression, thus producing obvious therapeutic effect on AD in rats.
研究雌激素对阿尔茨海默病（AD）大鼠模型脑组织中磷酸化Tau蛋白（P-Tau）、胆碱乙酰转移酶（ChAT）和神经生长因子（NGF）蛋白表达的影响。
通过向大鼠右侧脑室注射Aβ1-42蛋白片段建立AD大鼠模型。两周后，将17β-雌二醇片皮下植入大鼠颈部，并维持30天。分别采用HE染色和免疫组织化学方法观察大鼠脑神经元的病理变化以及P-Tau、ChAT和NGF蛋白表达的改变。
AD大鼠脑组织中出现神经原纤维缠结，雌激素治疗可显著减少神经原纤维缠结的形成。与AD模型大鼠相比，雌激素治疗还导致P-Tau表达降低，ChAT和NGF蛋白表达增加。
雌激素可上调ChAT和NGF表达，下调tau蛋白表达，从而对大鼠AD产生明显的治疗作用。
