Department of Molecular Microbiology, Cholera and Environmental Microbiology Laboratory, Rajiv Gandhi Centre for Biotechnology, Trivandrum, Kerala, India.
Curr Microbiol. 2011 Mar;62(3):758-63. doi: 10.1007/s00284-010-9774-3. Epub 2010 Oct 22.
Vibrio cholerae is causative agent of life threatening diarrheal disease, cholera. The toxin co-regulated pilus (TCP) is a critical colonization factor of V. cholerae and it also serves as receptor for CTXФ. In this study, we describe nucleotide sequence of four novel alleles of tcpA gene from toxigenic and non-toxigenic V. cholerae isolated from environmental sources. The phylogenetic analysis of tcpA revealed that it is related to tcpA of newly emerged O1 strain and unrelated to tcpA of wild type (classical and El Tor strains). All strains showed variant tcpA and also harbored intact Vibrio Pathogenicity Island (VPI). The expression of all variant alleles was demonstrated by RT-PCR.
霍乱弧菌是一种威胁生命的腹泻病，霍乱的病原体。毒素协同调节菌毛（TCP）是霍乱弧菌的一个关键定植因子，也是 CTXФ 的受体。在这项研究中，我们描述了从环境来源分离的产毒和非产毒霍乱弧菌中四个新的 tcpA 基因等位基因的核苷酸序列。tcpA 的系统发育分析表明，它与新出现的 O1 菌株的 tcpA 有关，而与野生型（经典和 El Tor 菌株）的 tcpA 无关。所有菌株均显示出变异的 tcpA，并且还具有完整的霍乱弧菌致病岛（VPI）。通过 RT-PCR 证明了所有变异等位基因的表达。
