Zheng Lin, Zhu Ling-Wei, Jing Jie, Guan Jia-Yao, Lu Ge-Jin, Xie Lin-Hong, Ji Xue, Chu Dong, Sun Yang, Chen Ping, Guo Xue-Jun
School of Food and Engineering, Jilin Agricultural University, Changchun, China.
The Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Changchun, China.
Front Vet Sci. 2021 May 31;8:638820. doi: 10.3389/fvets.2021.638820. eCollection 2021.
Migratory birds are recently recognized as disease vectors, but may be widespread transporters of strains. We isolated and strains from migratory bird epidemic samples from 2017 to 2018 and isolated from migratory bird feces in 2019 from bird samples taken from the Inner Mongolia autonomous region of China. To investigate the evolution of these two species, we sequenced the genomes of 40 strains and 34 strains isolated from the bird samples and compared these genomes with reference strain genomes. The pan-genome of all and genomes was large, with strains exhibiting considerable individual differences. A total of 2,130 and 1,352 core genes were identified in the and genomes, respectively, while dispensable genes accounted for 16,180 and 9,178 of all genes for the two strains, respectively. All strains isolated from the migratory birds that encoded T6SS and were non-O1/O139 serotypes without the ability to produce CTX. These strains also lacked the ability to produce the TCP fimbriae nor the extracellular matrix protein RbmA and could not metabolize trimetlylamine oxide (TMAO). Thus, these characteristics render them unlikely to be pandemic-inducing strains. However, a isolate encoding the complete T6SS system was isolated for the first time. These data provide new molecular insights into the diversity of . and isolates recovered from migratory birds.
候鸟最近被确认为疾病传播媒介，但可能是菌株的广泛传播者。我们从2017年至2018年的候鸟疫情样本中分离出了 和 菌株，并于2019年从中国内蒙古自治区采集的鸟类样本中的候鸟粪便中分离出了 。为了研究这两个 物种的进化，我们对从鸟类样本中分离出的40株 菌株和34株 菌株的基因组进行了测序，并将这些基因组与参考菌株基因组进行了比较。所有 和 基因组的泛基因组都很大，菌株表现出相当大的个体差异。在 和 基因组中分别鉴定出了2130个和1352个核心基因，而两个菌株的可 dispensable 基因分别占所有基因的16180个和9178个。从候鸟中分离出的所有编码T6SS和 的菌株均为非O1/O139血清型，无产生CTX的能力。这些菌株也缺乏产生TCP菌毛和细胞外基质蛋白RbmA的能力，且不能代谢氧化三甲胺（TMAO）。因此，这些特征使它们不太可能成为引发大流行的菌株。然而，首次分离出了一株编码完整T6SS系统的 菌株。这些数据为从候鸟中分离出的 和 菌株的多样性提供了新的分子见解。
