Department of Biotechnology, Faculty of Applied Sciences, UCSI University, Kuala Lumpur, Malaysia.
Anim Biotechnol. 2010 Oct;21(4):226-40. doi: 10.1080/10495398.2010.506334.
The present investigation was carried out in an attempt to study the phylogenetic analysis of different breeds of domestic chickens in Peninsular Malaysia inferred from partial cytochrome b gene information and random amplified polymorphic DNA (RAPD) markers. Phylogenetic analysis using both neighbor-joining (NJ) and maximum parsimony (MP) methods produced three clusters that encompassed Type-I village chickens, the red jungle fowl subspecies and the Japanese Chunky broilers. The phylogenetic analysis also revealed that majority of the Malaysian commercial chickens were randomly assembled with the Type-II village chickens. In RAPD assay, phylogenetic analysis using neighbor-joining produced six clusters that were completely distinguished based on the locality of chickens. High levels of genetic variations were observed among the village chickens, the commercial broilers, and between the commercial broilers and layer chickens. In this study, it was found that Type-I village chickens could be distinguished from the commercial chickens and Type-II village chickens at the position of the 27th nucleotide of the 351 bp cytochrome b gene. This study also revealed that RAPD markers were unable to differentiate the type of chickens, but it showed the effectiveness of RAPD in evaluating the genetic variation and the genetic relationships between chicken lines and populations.
本研究试图从部分细胞色素 b 基因信息和随机扩增多态性 DNA (RAPD) 标记中研究马来西亚半岛不同品种家鸡的系统发育分析。基于邻接法 (NJ) 和最大简约法 (MP) 的系统发育分析产生了 3 个聚类，其中包括 I 型乡村鸡、红原鸡亚种和日本 Chunky 肉鸡。系统发育分析还表明，大多数马来西亚商业鸡与 II 型乡村鸡随机混合。在 RAPD 分析中，基于邻接法的系统发育分析产生了 6 个聚类，这些聚类完全根据鸡的产地进行区分。乡村鸡、商业肉鸡以及商业肉鸡和蛋鸡之间存在高水平的遗传变异。在这项研究中，发现 I 型乡村鸡可以在 351bp 细胞色素 b 基因的第 27 个核苷酸位置与商业鸡和 II 型乡村鸡区分开来。这项研究还表明，RAPD 标记无法区分鸡的类型，但它显示了 RAPD 在评估鸡系和种群之间的遗传变异和遗传关系方面的有效性。
