Yan Linjun, She Yifan, Elzo Mauricio A, Zhang Chunlei, Fang Xingtang, Chen Hong
Institute of Cellular and Molecular Biology, Jiangsu Normal University, Xuzhou, Jiangsu 221116, China.
School of Environmental and Biological Engineering, Nantong College of Science and Technology, Nantong, Jiangsu 226007, China.
Arch Anim Breed. 2019 Jun 12;62(1):325-333. doi: 10.5194/aab-62-325-2019. eCollection 2019.
The objective of this research was to characterize the genetic diversity and phylogenetic diversity among 12 cattle breeds (10 Chinese breeds and two foreign taurine breeds as controls) utilizing gene mtDNA 16S rRNA. The complete sequences of the mtDNA 16S rRNA genes of the 251 animals were 1570 bp long. The mean percentages of the four nitrogen bases were 37.8 % for adenine (A), 23.7 % for thymine (T), 20.9 % for cytosine (C), and 17.6 % for guanine (G). The mtDNA 16S rRNA gene base percentages had a strong bias towards A T. All detected nucleotide variations in gene mtDNA 16S rRNA were either transitions (62.3 %) or transversions (37.7 %); no indels (insertions and deletions) were found. A total of 40 haplotypes were constructed based on these mutations. A total of 36 haplotypes of these 40 haplotypes were present in 10 Chinese cattle breeds. The haplotype diversity of all Chinese cattle populations was , while the nucleotide diversity was . Kimura's two-parameter genetic distances between pairs of the studied 12 breeds ranged from 0.001 to 0.010. The phylogenetic analysis assigned the 10 Chinese breeds to two distinct lineages that likely differed in their percentage of and ancestry.
本研究的目的是利用线粒体DNA 16S rRNA基因来表征12个牛品种（10个中国品种和2个作为对照的外国普通牛品种）的遗传多样性和系统发育多样性。251只动物的线粒体DNA 16S rRNA基因的完整序列长度为1570 bp。四种氮碱基的平均百分比分别为：腺嘌呤（A）37.8%、胸腺嘧啶（T）23.7%、胞嘧啶（C）20.9%、鸟嘌呤（G）17.6%。线粒体DNA 16S rRNA基因碱基百分比强烈偏向于A和T。在基因线粒体DNA 16S rRNA中检测到的所有核苷酸变异均为转换（62.3%）或颠换（37.7%）；未发现插入缺失（插入和缺失）。基于这些突变共构建了40个单倍型。在10个中国牛品种中存在这40个单倍型中的36个单倍型。所有中国牛群体的单倍型多样性为 （此处原文缺失具体数据）, 而核苷酸多样性为 （此处原文缺失具体数据）。所研究的12个品种之间的Kimura双参数遗传距离在0.001至0.010之间。系统发育分析将10个中国品种分为两个不同的谱系，它们在 （此处原文缺失具体数据）和 （此处原文缺失具体数据）血统的百分比上可能存在差异。
