Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing, PR China.
Electrophoresis. 2010 Nov;31(22):3665-74. doi: 10.1002/elps.201000258. Epub 2010 Oct 21.
Hybrid chips combing microchips with capillaries have displayed particular advantages in achieving UV-vis and mass spectroscopic detection. In this work, systematic 3-D numerical simulations have been carried out to explore the influence of junction interface cross-sectional area and ζ-potential distribution on sample band broadening in hybrid-chip electrophoresis separation. In this case, the ratio of cross-sectional area of chip to capillary channel (S(ratio) ) is used as the parameter of the variation in junction interface cross-sectional area. Theoretical simulations demonstrated that the decrease of the S(ratio) would increase the separation efficiency in the hybrid-chip-based CE with uniform ζ-potential distribution. ζ-potential distribution along the axial direction of the channel also affects mass transport in hybrid-chip-based CE. Therefore, the effect of ζ-potential distribution has been considered in the 3-D simulation. Theoretical simulation results reveal that ζ-potential distribution rather than the interface cross-sectional area variation (S(ratio) ) controls the sample band broadening and manipulates sample separation efficiency in the hybrid-chip-based CE with non-uniform ζ-potential distribution. Both the theoretical simulations and experimental results show that optimal hybrid-chip CE separation efficiency can be achieved at S(ratio) =1.
杂交芯片将微芯片与毛细血管结合在一起，在实现紫外可见和质谱检测方面显示出了特殊的优势。在这项工作中，我们进行了系统的三维数值模拟，以探讨在杂交芯片电泳分离中，连接界面的截面积和 ζ 电势分布对样品带展宽的影响。在这种情况下，我们使用芯片与毛细管通道的截面积比 (S(ratio) ) 作为连接界面截面积变化的参数。理论模拟表明，在具有均匀 ζ 电势分布的杂交芯片 CE 中，减小 S(ratio) 会增加分离效率。 ζ 电势沿通道轴向的分布也会影响杂交芯片 CE 中的质量传输。因此，在三维模拟中考虑了 ζ 电势分布的影响。理论模拟结果表明，在具有非均匀 ζ 电势分布的杂交芯片 CE 中，控制样品带展宽并操纵样品分离效率的是 ζ 电势分布，而不是界面截面积变化 (S(ratio) )。理论模拟和实验结果均表明，在 S(ratio) =1 时可以实现最佳的杂交芯片 CE 分离效率。
