Jadraque María, Monforte Agustín, Nuñez María Teresa, López-Gómez Laura, Martín Margarita, Serrano Fernando
Instituto de Química-Física Rocasolano, CSIC, Madrid, Spain.
Photomed Laser Surg. 2011 Feb;29(2):75-81. doi: 10.1089/pho.2010.2769. Epub 2010 Oct 22.
Development of a robotic ArF excimer laser device with a three-dimensional (3D) pattern scanning sensor for the controlled de-epithelization of live mouse and xenografted epidermis.
The animal model could be adapted to humans for automated, minimally invasive de-epithelization of cutaneous areas and therefore is of interest for cutaneous gene therapy research.
Ablation thresholds of mouse, porcine, and human skin were measured by acoustic detection methods. These ablation thresholds were used as initial parameters for dosimetry measurements. De-epithelization of live mouse and xenografted epidermis was performed by laser ablation (ArF excimer laser, λ = 193 nm, t(p) = 20 nsec). The rectangular shape of the laser spot and a robotic arm displacement incorporating a three-dimensional patter scanning sensor allowed a polygonal tile floor irradiation of a 2-cm-diameter area. Ablated epidermis was subjected to histology.
SCID and nude mouse skin did not entirely reflect the de-epithelization of human skin because abundant pockets of dermal keratinocytes persist in the outer root sheath of hair and cysts providing competitive foci of re-epithelization. Automated de-epithelization of human and porcine skin xenografts resulted in precise removal of keratinocytes with subcellular precision, providing a smooth live surface where epidermal transplants might engraft with little endogenous competition from residual outer root sheath from rare hairs.
The displacement of the ArF excimer laser devices allows reproducible, smooth, and damage-free ablation of epidermal areas in the animal model.
开发一种带有三维（3D）图案扫描传感器的机器人氩氟准分子激光设备，用于对活小鼠和异种移植表皮进行可控的上皮去除。
该动物模型可适用于人类，用于皮肤区域的自动化、微创上皮去除，因此对皮肤基因治疗研究具有重要意义。
通过声学检测方法测量小鼠、猪和人类皮肤的消融阈值。这些消融阈值用作剂量测定测量的初始参数。通过激光消融（氩氟准分子激光，λ = 193 nm，t(p) = 20纳秒）对活小鼠和异种移植表皮进行上皮去除。激光光斑的矩形形状以及结合三维图案扫描传感器的机械臂位移允许对直径2厘米的区域进行多边形平铺照射。对消融的表皮进行组织学检查。
严重联合免疫缺陷（SCID）小鼠和裸鼠皮肤不能完全反映人类皮肤的上皮去除情况，因为在毛囊外根鞘和囊肿中存在大量真皮角质形成细胞囊，这些囊提供了竞争性的再上皮化焦点。对人类和猪皮肤异种移植物进行自动化上皮去除可实现角质形成细胞的精确去除，具有亚细胞精度，提供了一个光滑的活表面，表皮移植在此处可能植入，且几乎没有来自罕见毛发残留外根鞘的内源性竞争。
氩氟准分子激光设备的位移可在动物模型中实现对表皮区域的可重复、平滑且无损伤的消融。
